anti-Laminin gamma 1 rat monoclonal, A5, purified

Cat. No. 691527

Available, delivery time 1-7 days

€340.00*

Key Features
  • Rat monoclonal
  • Suitable for FACS, ICC/IF, IHC and WB
  • Reacts with human, monkey, mouse, pig and rat
  • Isotype: IgG2a kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Rat
Isotype IgG2a kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Human, Monkey, Mouse, Pig, Rat
Storage 2-8°C
Intended use Research use only
Clone A5
Immunogen Laminin preparation from murine EHS cells
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P11047 (Human), P02468 (Mouse), A0A4X1TYI6 (Pig), F1MAA7 (Rat)
Synonym Laminin subunit gamma-1, Laminin B2 chain, Laminin-1 subunit gamma, Laminin-10 subunit gamma, Laminin-11 subunit gamma, Laminin-2 subunit gamma, Laminin-3 subunit gamma, Laminin-4 subunit gamma, Laminin-6 subunit gamma, Laminin-7 subunit gamma, Laminin-8 subunit gamma, Laminin-9 subunit gamma, S-laminin subunit gamma, S-LAM gamma, LAMC1, LAMB2
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Western Blot (WB) 1:50-1:100 (1-2 µg/ml)
Background

Laminins are large hetero-trimeric, non-collagenous glycoproteins found in basement membranes and composed of alpha, beta, and gamma chains. A5 reacts specifically with gamma chain 1. Alterations of basement membrane integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.

Positive control: normal colon or carcinomas.

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