Highly published

anti-Laminin rabbit polyclonal, serum

Cat. No. 10765

Available, delivery time 1-7 days


Key Features
  • Rabbit polyclonal
  • Suitable for IHC on frozen and paraffin sections and WB
  • Reacts with bovine, human, mouse, pig, rabbit and rat
Product description
Quantity 250 µl
Antibody Type Polyclonal
Host Rabbit
Conjugate Unconjugated
Application IHC, WB
Purification Stabilized antiserum
Reactivity Bovine, Human, Mouse, Pig, Rabbit, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Laminin isolated from Engelbrecht-Holm-Swarm (EHS)-mouse sarcoma
Formulation Contains 0.09% sodium azide
UniprotID A0A3Q1LUC5 (Bovine),P25391 (Human),P19137 (Mouse),A0A4X1VLN1 (Pig),H0VND7 (Guinea pig),G1TTH9 (Rabbit),G1TTH9 (Rabbit),D4A409 (Rat)
Synonym Laminin subunit alpha-1, Laminin A chain, Laminin-1 subunit alpha, Laminin-3 subunit alpha, S-laminin subunit alpha, S-LAM alpha, LAMA1, LAMA
Note Centrifuge prior to opening
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:50-1:100
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (proteolytic treatment required; enzyme: pepsin)
Western Blot (WB) Assay dependent

Laminin is a glycoprotein (Mr 850 - 1.000 kD, consisting of 3 glycosylated polypeptide chains with molecular weights of 440 and 225 (2x) kD) produced by various human epithelial and mesenchymal cells, and forms an extracellular matrix of thin filaments. In normal tissues, laminin is invariably present in all basal laminas surrounding muscle, nerve, fat and decidua cells and separates epithelial and endothelial cells from abutting connective tissues. Laminin has also been identified within the cytoplasm of breast epithelia, stromal cells of the endometrium, and within endothelial, bile duct epithelial and mesenchymal cells of the liver. Laminin has been found to be involved in cellular activities such as adhesion, spreading, differentiation, polarization, proliferation, locomotion, tissue invasion and chemotactic responses.
No cross reaction was obtained with human type I, III, IV and V collagen in immunoblotting, whereas the antibody reacted with a distinct band of appr. 200-220 kD from a 8M Urea extract from amnion basement membrane.

Positive control: skin, kidney.

References/Publications (7)
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The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

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