anti-Leukocyte Common Antigen (CD45) mouse monoclonal, BRA-55, ascites

Cat. No. 10914

Available, delivery time 1-7 days

€177.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC on frozen and paraffin sections and WB
  • Reacts with human
  • Isotype: IgG1
Product description
Quantity 1 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Ascites
Reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone BRA-55
Immunogen Isolated from REH6 cell line
Formulation Contains 0.09% sodium azide
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:10-1:20
Immunohistochemistry (IHC) - paraffin 1:10-1:20 (microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

The antibody reacts with 170 - 220 kD cell surface glycoproteins (CD45), the Leukocyte Common Antigen (LCA), expressed selectively on hematopoietic cells. It gives a pronounced staining on formalin-fixed, paraffin-embedded cells, and can be used to differentiate between lymphomas and carcinomas.

Positive control: Tonsil.

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