anti-Lewis b mouse monoclonal, 2-25LE, purified

Cat. No. 691509

Available, delivery time 1-7 days

€340.00*

Key Features
  • Mouse monoclonal
  • Suitable for ELISA, FACS, ICC/IF, IHC and WB
  • Reacts with guinea pig and human
  • Isotype: IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application ELISA, FACS, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Guinea pig, Human
Storage 2-8°C
Intended use Research use only
Clone 2-25LE
Immunogen ALea-b+ ovarian cyst mucin
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P21217 (Human)
Synonym 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase FUT3, EC 2.4.1.65, Alpha-3-fucosyltransferase FUT3, EC 2.4.1.-, Blood group Lewis alpha-4-fucosyltransferase, Lewis FT, Fucosyltransferase 3, Fucosyltransferase III, FucT-III, FUT3, FT3B, LE
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
ELISA Assay dependent
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Western Blot (WB) 1:50-1:100 (1-2 µg/ml)
Background

2-25LE reacts with Lewis b antigen which is highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver.

Positive control: stomach, colon, small intestine, lung and kidney, colorectal adenocarcinoma.

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