Epitope mapped

anti-M13/fd/F1 Filamentous Phages mouse monoclonal, B62-FE2, lyophilized, purified, 100 µg

Lyophilized

Lyophilized

HRP

HRP

Biotin

FITC

Cat. No. 61097

Available, delivery time 1-7 days

€178.00*

Key Features
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ELISA, phage display, IEM and WB
  • Reacts with filamentous phages F1, filamentous phages fd and filamentous phages M13
  • Isotype: IgG2b
Product description
Quantity 100 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG2b
Conjugate Unconjugated
Application ELISA, IEM, Phage display, WB
Purification Affinity chromatography
Reactivity Filamentous phages F1, Filamentous phages M13, Filamentous phages fd
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone B62-FE2
Immunogen Fd phages from E. coli F+ strain (JM109)
Concentration 100 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Synonym M13
Applications
Tested applications Tested dilutions
ELISA 1:1,000
Immuno Electron Microscopy (IEM) Assay dependent
Phage Display Assay dependent (detection limit: 1E+07 phage particles)
Western Blot (WB) Assay dependent
Background

B62-FE2 binds to an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT (Kneissel et al. 1999).

Kneissel, S. et al. Epitope structures recognised by antibodies against the major coat protein (g8p) of filamentous bacteriophage fd (Inoviridae). J. Mol. Biol. 288, 21-8 (1999).

References/Publications (3)
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