anti-MALT1 mouse monoclonal, EBS-T-007, purified
- Mouse monoclonal
- Suitable for IHC and WB
- Reacts with human
- Isotype: IgG1 kappa
|Quantity||1 ml (100 µg/ml)|
|Intended use||Research use only|
|Immunogen||Human MALT1 recombinant protein|
|Formulation||PBS with 0.02% sodium azide|
|Synonym||Mucosa-associated lymphoid tissue lymphoma translocation protein 1, EC 3.4.22.-, MALT lymphoma-associated translocation, Paracaspase, MALT1, MLT|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:50-1:100 (1-2 µg/ml)|
|Immunohistochemistry (IHC) - paraffin||1:50-1:100 (1-2 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 recommended)|
|Western Blot (WB)||1:50-1:100 (1-2 µg/ml)|
EBS-T-007 reacts with paracaspase MALT1, crucial for B- and T-cell activation/proliferation and activation of transcription factor NF-kappaB. It has a N-terminal death domain, two central immunoglobulin-like domains involved in the binding to the B-cell lymphoma 10 (BCL10) protein and a caspase-like domain. MALT1 and BCL10 can both be found translocated in MALT lymphoma. In such cases either BCL10 or MALT1 or both are highly expressed, depending on the site of translocation. Normal cells and MALT lymphomas lacking translocations exhibit much lower levels of expression.
Positive control: HepG2 cells or lymphoma.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
- Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
- Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
- Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
- Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.