anti-MUC2 mouse monoclonal, EBS-T-233, purified

Cat. No. 691679

Available, delivery time 1-7 days

€340.00*

Key Features
  • Mouse monoclonal
  • Suitable for FACS, ICC/IF, IHC and WB
  • Reacts with human
  • Isotype: IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Human
Storage 2-8°C
Intended use Research use only
Clone EBS-T-233
Immunogen Synthetic human MUC2 peptide (VNTR region)
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID Q02817 (Human)
Synonym Mucin-2, MUC-2, Intestinal mucin-2, MUC2, SMUC
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 10 mM Tris with 1 mM EDTA, pH 9.0, recommended)
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Western Blot (WB) 1:50-1:100 (1-2 µg/ml)
Background

EBS-T-233 reacts with GTQTP in the VNTR domain of human MUC2 (520 kDa). MUC2 is specifically expressed in goblet cells of the small intestine and colon and about 65% of colonic carcinomas and about 40% of gastric carcinomas are positive. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.

Positive control: LS174T cells (flow cytometry, Western blot), small intestine.

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