anti-MUC3 mouse monoclonal, EBS-T-232, purified

Cat. No. 691678

Available, delivery time 1-7 days

€340.00*

Key Features
  • Mouse monoclonal
  • Suitable for FACS, ICC/IF and IHC
  • Reacts with human
  • Isotype: IgG2a kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC
Purification Affinity chromatography
Reactivity Human
Storage 2-8°C
Intended use Research use only
Clone EBS-T-232
Immunogen Synthetic human MUC3 (SIB-35) peptide
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID Q02505 (Human)
Synonym Mucin-3A, MUC-3A, Intestinal mucin-3A, MUC3A, MUC3
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 10 mM Tris with 1 mM EDTA pH 9.0 recommended)
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Background

EBS-T-232 reacts with SITTTE in the VNTR domain of human MUC3. The mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (MUC1), 69 base pairs (MC2) and 51 base pairs (MUC3). Cancer cells of colon, breast and stomach, normal cells of salivary gland, breast, lung, and gastrointestinal tract are positive for MUC3.

Positive control: LS174T cells or colon carcinoma and normal colon tissue.

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