Disease related

anti-Melanoma-Associated Antigen mouse monoclonal, NKI/C-3, ascites fluid

Cat. No. 10819

Available, delivery time 1-7 days


Key Features
  • Mouse monoclonal
  • Suitable for ELISA, IHC on paraffin sections and WB
  • Reacts with human
  • Isotype: IgG1
Product description
Quantity 1 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ELISA, IHC, WB
Purification Ascites
Reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone NKI/C-3
Immunogen Isolated from human melanoma cells
Formulation Contains 0.09% sodium azide
Note Centrifuge prior to opening
Tested applications Tested dilutions
Immunohistochemistry (IHC) - paraffin 1:5-1:10 (microwave treatment recommended)
ELISA Assay dependent
Western Blot (WB) Assay dependent

NKI/C-3 recognizes a melanoma-associated antigen present in the cytoplasm of melanoma cells and nevocellular nevi, in contrast to most mabs against melanoma-associated antigens present in the membrane, which are not retained after formalin fixation. Therefore, the mab is very useful for paraffin sections in routine pathology. Cross reactivity with carcinoids, medullary carcinomas of the thyroid, and occasionally prostatic, primary breast, avarian, and lung carcinomas may be observed.
No reaction is seen with basal cell carcinomas or brain tumors. NKI/C-3 does not react with normal melanocytes or other normal tissues except for mastcells, histiocytes, salivary glands, bronchial glands, pancreatic und prostatic epithelium.
For the diagnosis of melanoma, the antibody should be used in combination with other antibodies (e.g. cytokeratin immunoreactivity indicative for carcinoma versus vimentin immunoreactivity indicative for melanoma).
In immunoblotting the antibody reacts with diffuse protein bands between 25 and 100 kD.
Positive control: Melanoma.

References/Publications (1)
Q & A's

Please enter a valid email address.
Please enter a valid question.
Fields marked with * are mandatory.

There aren't any asked questions yet.
Customer Reviews

0 of 0 reviews

Leave a review!

Share your experiences with other customers.

The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.