anti-Myosin mouse monoclonal, 414.0, supernatant

Cat. No. 10605

Available, delivery time 1-7 days

€273.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC on frozen and paraffin sections and WB
  • Reacts with chicken, human, mouse and rabbit
  • Isotype: IgM
Product description
Quantity 1 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgM
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Chicken, Human, Mouse, Rabbit
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone 414.0
Immunogen Chicken skeletal muscle cell preparation
Formulation Contains 0.09% sodium azide
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:5-1:10
Immunohistochemistry (IHC) - frozen 1:5-1:10
Immunohistochemistry (IHC) - paraffin 1:5-1:10 (microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

Mab 414 reacts with myosin-containing elements in most cell types. Antigen localization: cytoplasm In Western blot experiments the antibody reacts with a 75kD part of the heavy chain (epitope location on a myosin-common site-chain), no reaction with light chains.
It is not reactive with alpha-cardiac myosin.

Positive control: muscle, myofibrils (chicken)

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