Epitope mapped

anti-N-CAM mouse monoclonal, RNL-1, supernatant

Cat. No. 11421

Available, delivery time 1-7 days


Key Features
  • Mouse monoclonal
  • Suitable for IHC on frozen sections and WB
  • Reacts with human
  • Isotype: IgG1
Product description
Quantity 1 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone RNL-1
Immunogen Cytoskeletal preparation of human epidermis
Formulation Contains 0.09% sodium azide
UniprotID P13591 (Human)
Synonym Neural cell adhesion molecule 1, N-CAM-1, NCAM-1, CD antigen CD56, NCAM1, NCAM
Note Centrifuge prior to opening
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10-1:20
Western Blot (WB) Assay dependent

Mab RNL-1, directed against N-CAM (Neural Cell Adhesion Molecule), reacts with normal neural tissues and endocrine glands, such as pancreatic islet, pituitary gland and adrenal medulla. Expression was also found in Leydig cells of the testis, in the thyroid and in smooth-muscle cells of the small intestine, colon and bladder. The antibody is a valuable marker for the characterization of several neuroendocrine tumors. In immunoblotting (Western) the antibody is reactive with 3 main clusters of protein bands in the molecular weight region of 200 kD, 100 kD, and 25-27 kD.

Positive control: Pancreatic islet cells (cell surface staining).

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The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.