anti-Nephrin guinea pig polyclonal, serum

Cat. No. GP-N2

Available, delivery time: 1-7 days

€289.00*

Key Features

Guinea pig polyclonal
Suitable for ICC/IF, IHC and WB
Reacts with human and mouse

Product description

Quantity	100 µl
Antibody Type	Polyclonal
Host	Guinea pig
Conjugate	Unconjugated
Application	ICC/IF, IHC, WB
Purification	Stabilized antiserum
Reactivity	Human, Mouse
Storage	Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use	Research use only
Immunogen	Synthetic peptide of mouse Nephrin (intracellular domain, aa1243-1256, EPGSLPFELRGHLV)
Formulation	Contains 0.09% sodium azide
UniprotID	O60500 (Human), Q9QZS7 (Mouse)
Synonym	Nephrin, Renal glomerulus-specific cell adhesion receptor, NPHS1, NPHN
Note	Centrifuge prior to opening

Applications

Tested applications	Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	Assay dependent
Immunohistochemistry (IHC) - frozen	1:50
Immunohistochemistry (IHC) - paraffin	1:50 (microwave treatment recommended)
Western Blot (WB)	1:500

Background

The antibody reacts specifically with nephrin, first described as a kidney podocyte marker protein (MW 135,000 calculated from aa sequence data; apparent Mr 185,000 after SDS-PAGE).
Nephrin is a transmembrane cell adhesion molecule located at the slit diaphragm.
Immunolocalization: the antibody stains positively podocytes of the kidney, radial glial cells of the brain, Sertoli cells of testis, and beta islet cells of the pancreas.
Tested cultured cell lines: PCL (podocyte cell line), M-1 (cortical collecting duct cells).

References/Publications (106)

Publication

Species

Application

Publication Chen, D. et al. Characterization of glomerular basement membrane components within pediatric glomerular diseases. Clin Kidney J 17, 37 (2024).

Species human

Publication Komatsu, S. et al. Detecting and exploring kidney-derived extracellular vesicles in plasma. Clin Exp Nephrol 28, 617–628 (2024).

Species human

Application IHC

Publication Carpenter, J., Yarlagadda, S., VandenHeuvel, K. A., Ding, L. & Schuh, M. P. Human Nephrogenesis can Persist Beyond 40 Postnatal Days in Preterm Infants. Kidney Int Rep 9, 436–450 (2024).

Species human

Publication Susa, K. et al. ATP/ADP biosensor organoids for drug nephrotoxicity assessment. Front Cell Dev Biol 11, (2023).

Application IHC

Publication Lian, E., Pietrobon, A. & Stanford, W. L. Differentiation and single-cell RNA-seq analyses of human pluripotent-stem-cell-derived renal organoids. STAR Protoc 4, (2023).

Species human

Application ICC-IF

Publication Priante, G. et al. Emerging Perspectives on the Rare Tubulopathy Dent Disease: Is Glomerular Damage a Direct Consequence of ClC-5 Dysfunction? Int J Mol Sci 24, (2023).

Species human

Publication Barutta F. et al., Protective effect of the tunneling nanotube-TNFAIP2/M-sec system on podocyte autophagy in diabetic nephropathy., Autophagy, 19, 505-524, (2023).

Species Mouse

Application IHC-IF

Publication Chung, H. et al. Curcumin Blocks High Glucose-Induced Podocyte Injury via RIPK3-Dependent Pathway. Front Cell Dev Biol 10, (2022).

Species mouse

Application WB

Publication Mousseaux, C. et al. Heterozygous expression of Cre recombinase in podocytes has no impact on the anti-glomerular basement membrane glomerulonephritis model in C57BL/6J mice. Physiol Rep 10, (2022).

Species mouse

Application IHC-IF

Publication Saito, Y., Matsumoto, N., Yamanaka, S., Yokoo, T. & Kobayashi, E. Beneficial Impact of Interspecies Chimeric Renal Organoids Against a Xenogeneic Immune Response. Front. Immunol. 13, (2022).

Species mouse

Application ICC-IF

Publication Kocylowski, M. K. et al. A slit-diaphragm-associated protein network for dynamic control of renal filtration. Nat. Commun. 13, 1–15 (2022).

Species Mouse

Application IF

Publication Ha, K. B. et al. EW-7197 Attenuates the Progression of DiabetiHa, K. B. et al. EW-7197 Attenuates the Progression of Diabetic Nephropathy in db/db Mice through Suppression of Fibrogenesis and Inflammation. Endocrinol. Metab. 37, 96–111 (2022).

Species Mouse

Application WB

Publication Bensaada, I. et al. Calpastatin prevents Angiotensin II–mediated podocyte injury through maintenance of autophagy. Kidney Int 100, 90–106 (2021).

Application IHC

Publication Zhang, S. Y. et al. CMIP interacts with WT1 and targets it on the proteasome degradation pathway. Clin Transl Med 11, (2021).

Species mouse

Application IHC

Publication Namba, M. et al. Creation of X-linked Alport syndrome rat model with Col4a5 deficiency. Sci Rep 11, 20836 (2021).

Species rat

Application IHC(paraffin)-IF

Publication Cohen, C. et al. Glomerular endothelial cell senescence drives age‐related kidney disease through PAI‐1. EMBO Mol Med 13, (2021).

Species mouse

Application IHC-IF

Publication Ohmori, T. et al. Impaired NEPHRIN localization in kidney organoids derived from nephrotic patient iPS cells. Sci Rep 11, 3982 (2021).

Species human

Application ICC-IF

Publication Makino, S. I. et al. Impairment of proteasome function in podocytes leads to CKD. Journal of the American Society of Nephrology 32, 597–613 (2021).

Species mouse

Application WB, IHC

Publication Okabe, M. et al. Indirect podocyte injury manifested in a partial podocytectomy mouse model. Am J Physiol Renal Physiol 320, F922–F933 (2021).

Species mouse

Application IHC-IF

Publication Maier, J. I. et al. A novel model for nephrotic syndrome reveals associated dysbiosis of the gut microbiome and extramedullary hematopoiesis. Cells 10, (2021).

Species mouse

Application IHC-IF (paraffin)

Publication Bhargava, R. et al. Aberrantly glycosylated IgG elicits pathogenic signaling in podocytes and signifies lupus nephritis. JCI Insight 6, (2021).

Species human

Application WB, IHC-IF

Publication Majmundar, A.J. et al. Recessive NOS1AP variants impair actin remodeling and cause glomerulopathy in humans and mice. Sci Adv. 7, NULL(2021).

Species rat

Application IHC (frozen)/IF

Publication Schreiber, J. et al. Bet proteins regulate expression of osr1 in early kidney development. Biomedicines 9, (2021).

Species mouse

Application IHC-IF

Publication Tesch, F. et al. Super-resolved local recruitment of CLDN5 to filtration slits implicates a direct relationship with podocyte foot process effacement. J. Cell. Mol. Med. 25, 7631–7641 (2021).

Species human

Application IHC-IF

Publication Maier, J.I. et al. EPB41L5 controls podocyte extracellular matrix assembly by adhesome-dependent force transmission. Cell Rep. 34, 108883(2021).

Species mouse

Application IHC (frozen)/IF

Publication Yan, Q. et al. A negative feedback loop between JNK-associated leucine zipper protein and TGF-β1 regulates kidney fibrosis. Commun Biol 3, 1–16 (2020).

Species mouse

Publication Satyam, A., Tsokos, M. G., Tresback, J. S., Zeugolis, D. I. & Tsokos, G. C. Cell-Derived Extracellular Matrix-Rich Biomimetic Substrate Supports Podocyte Proliferation, Differentiation, and Maintenance of Native Phenotype. Adv Funct Mater 30, (2020).

Species human

Application WB

Publication Groener, M. et al. Identification of Podocyte Cargo Proteins by Proteomic Analysis of Clathrin-Coated Vesicles. Kidney360 1, 480–490 (2020).

Species mouse

Publication Tang, P., Das, J. R., Li, J., Yu, J. & Ray, P. E. An HIV-Tat inducible mouse model system of childhood HIV-associated nephropathy. DMM Dis. Model. Mech. 13, (2020).

Species mouse

Application WB

Publication Tian, X. et al. Inhibiting calpain 1 and 2 in cyclin G associated kinase–knockout mice mitigates podocyte injury. JCI Insight 5, (2020).

Species human

Application IHC-IF

Publication Son, S.S., Kang, J. & Lee, E. Paclitaxel Ameliorates Palmitate-Induced Injury in Mouse Podocytes. Med Sci Monit Basic Res. 26, e928265(2020).

Species mouse

Application WB

Publication Hayashi, D. et al. The mechanisms of ameliorating effect of a green tea polyphenol on diabetic nephropathy based on diacylglycerol kinase α. Sci.Rep. 10, 11790 (2020)

Species mouse

Application IHC-IF

Publication Ettou, S. et al. Epigenetic transcriptional reprogramming by WT1 mediates a repair response during podocyte injury. Sci Adv. 6, eabb5460(2020).

Species rat

Application IHC/IF

Publication Wang, Q. et al. Protective role of tangshen formula on the progression of renal damage in db/db mice by TRPC6/Talin1 pathway in podocytes. J. Diabetes Res. 2020, (2020).

Species mouse

Application WB, IHC-IF (paraffin)

Publication Kang, J. S. et al. Angiotensin II-mediated MYH9 downregulation causes structural and functional podocyte injury in diabetic kidney disease. Sci Rep 9, (2019).

Species human, mouse

Application WB

Publication Low, J. H. et al. Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network. Cell Stem Cell 25, 373-387.e9 (2019).

Species human

Application ICC-IF

Publication Wanner, N. et al. DNA Methyltransferase 1 Controls Nephron Progenitor Cell Renewal and Differentiation. J.Am.Soc.Nephrol. 30, 63-78 (2019)

Species mouse

Application IHC-IF (paraffin)

Publication Inoue, K. et al. Podocyte histone deacetylase activity regulates murine and human glomerular diseases. J.Clin.Invest. 129, 1295-1313 (2019)

Species mouse

Application IHC-IF (paraffin)

Publication Murakami, Y. et al. Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation. Sci.Rep. 9, 1172 (2019)

Species mouse

Application IHC-IF (paraffin)

Publication Hishikawa, A. et al. Decreased KAT5 Expression Impairs DNA Repair and Induces Altered DNA Methylation in Kidney Podocytes. Cell.Rep. 26, 1318-1332.e4 (2019)

Species human

Application ICC-IF

Publication Zhao, J. et al. CD36-Mediated Lipid Accumulation and Activation of NLRP3 Inflammasome Lead to Podocyte Injury in Obesity-Related Glomerulopathy. Mediators.Inflamm. 2019, 3172647 (2019)

Species mouse

Application IHC-IF

Publication Brinkkoetter, P. et al. Anaerobic Glycolysis Maintains the Glomerular Filtration Barrier Independent of Mitochondrial Metabolism and Dynamics. Cell.Rep. 27, 1551-1566.e5 (2019)

Species mouse

Application IHC-IF (paraffin)

Publication Okabe, M. et al. Global polysome analysis of normal and injured podocytes. Am.J.Physiol.Renal.Physiol. 316, F241-F252 (2019)

Species mouse

Application IHC

Publication Lee, S. et al. CCR2 knockout ameliorates obesity-induced kidney injury through inhibiting oxidative stress and ER stress. PLoS.One. 14, e0222352 (2019)

Species mouse

Application WB

Publication Denhez, B. et al. Diabetes-Induced DUSP4 Reduction Promotes Podocyte Dysfunction and Progression of Diabetic Nephropathy. Diabetes. 68, 1026-1039 (2019)

Species mouse

Application IHC-IF (frozen)

Publication Maeda, K. et al. CaMK4 compromises podocyte function in autoimmune and nonautoimmune kidney disease. J.Clin.Invest. 128, 3445-3459 (2018).

Species mouse

Application FACS

Publication Musah, S., Dimitrakakis, N., Camacho, D. M., Church, G. M. & Ingber, D. E. Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip. Nat Protoc 13, 1662–1685 (2018).

Species human

Application IHC(frozen)

Publication Itoh, M., Nakadate, K., Matsusaka, T., Hunziker, W. & Sugimoto, H. Effects of the differential expression of ZO-1 and ZO-2 on podocyte structure and function. Genes to Cells 23, 546–556 (2018).

Species mouse

Application IHC(frozen)-IF

Publication Ahadzadeh, E. et al. The chemokine receptor CX3CR1 reduces renal injury in mice with angiotensin II-induced hypertension. Am.J.Physiol.Renal.Physiol. 315, F1526-F1535 (2018)

Species mouse

Application IHC (paraffin)

Publication Siegerist, F. et al. Novel Microscopic Techniques for Podocyte Research. Front.Endocrinol.(Lausanne). 9, 379 (2018).

Species mouse,rat

Application IHC-IF (paraffin)

Publication Hermle, T. et al. GAPVD1 and ANKFY1 Mutations Implicate RAB5 Regulation in Nephrotic Syndrome. J.Am.Soc.Nephrol. 29, 2123-2138 (2018)

Species human

Application IHC-IF (paraffin)

Publication Lenoir, O. et al. Hmox1 Deficiency Sensitizes Mice to Peroxynitrite Formation and Diabetic Glomerular Microvascular Injuries. J.Diabetes.Res. 2017, 9603924 (2018).

Species mouse

Application IHC (paraffin)

Publication Ruhnke, L. et al. Progenitor Renin Lineage Cells are not involved in the regeneration of glomerular endothelial cells during experimental renal thrombotic microangiopathy. PLoS.One. 13, e0196752 (2018).

Species mouse

Application IHC-IF (paraffin)

Publication Dorotea, D. et al. Orally active, species-independent novel A3 adenosine receptor antagonist protects against kidney injury in db/db mice. Exp.Mol.Med. 50, 38 (2018).

Species mouse

Application IHC (paraffin)

Publication Rauch, C. et al. Differentiation of human iPSCs into functional podocytes. PLoS.One. 13, e0203869 (2018).

Species human

Application WB

Publication Uchida, T. et al. Repeated administration of alpha-galactosylceramide ameliorates experimental lupus nephritis in mice. Sci.Rep. 8, 8225 (2018).

Species mouse

Application IHC-IF (paraffin)

Publication Artelt, N. et al. Comparative Analysis of Podocyte Foot Process Morphology in Three Species by 3D Super-Resolution Microscopy. Front.Med.(Lausanne). 5, 292 (2018).

Species human,mouse,rat

Application IHC (paraffin)

Publication Ueda, Y. et al. Blocking Properdin Prevents Complement-Mediated Hemolytic Uremic Syndrome and Systemic Thrombophilia. J.Am.Soc.Nephrol. 29, 1928-1937 (2018)

Species mouse

Application IHC-IF (frozen)

Publication Tanigawa, S. et al. Organoids from Nephrotic Disease-Derived iPSCs Identify Impaired NEPHRIN Localization and Slit Diaphragm Formation in Kidney Podocytes. Stem.Cell.Reports. 11, 727-740 (2018).

Species human

Application IHC-IF (frozen)

Publication Luo, W. et al. Alternative Pathway Is Essential for Glomerular Complement Activation and Proteinuria in a Mouse Model of Membranous Nephropathy. Front.Immunol. 9, 1433 (2018).

Species mouse

Application IHC-IF (frozen)

Publication Armelloni, S. et al. NeuroD Expression in Podocytes and Interrelationships with Nephrin at Both Nuclear and Cytoplasmic Sites. Cell.Physiol.Biochem. 46, 873-889 (2018)

Species human,mouse

Application WB

Publication Schell, C. et al. ARP3 Controls the Podocyte Architecture at the Kidney Filtration Barrier. Dev Cell. 47, 741-757.e8(2018).

Species human

Application IHC (paraffin)

Publication Ma, Y. et al. Role of c-Abl and nephrin in podocyte cytoskeletal remodeling induced by angiotensin II. Cell.Death.Dis. 9, 185 (2018).

Species grivet monkey

Application WB

Publication Kliewe, F. et al. Studying the role of fascin-1 in mechanically stressed podocytes. Sci.Rep. 7, 9916 (2017).

Species human,mouse

Application IHC-IF (paraffin)

Publication Henique, C. et al. Genetic and pharmacological inhibition of microRNA-92a maintains podocyte cell cycle quiescence and limits crescentic glomerulonephritis. Nat.Commun. 8, 1829 (2017).

Species human,mouse

Application IHC-IF (paraffin)

Publication Taguchi, A and Nishinakamura, . Higher-Order Kidney Organogenesis from Pluripotent Stem Cells. Cell.Stem.Cell. 21, 730-746.e6 (2017)

Species mouse

Application whole mount

Publication Lin, J. et al. ARF6 mediates nephrin tyrosine phosphorylation-induced podocyte cellular dynamics. PLoS.One. 12, e0184575 (2017).

Species human

Application IHC-IF (paraffin)

Publication Kaku, Y. et al. PAX2 is dispensable for in vitro nephron formation from human induced pluripotent stem cells. Sci. Rep. 7, 1-12 (2017)

Species human

Application ICC-IF

Publication Dumont, V. et al. PACSIN2 accelerates nephrin trafficking and is up-regulated in diabetic kidney disease. FASEB.J. 31, 3978-3990 (2017).

Species human,mouse

Application WB

Publication Siegerist, F. et al. Structured illumination microscopy and automatized image processing as a rapid diagnostic tool for podocyte effacement. Sci.Rep. 7, 11473 (2017).

Species human

Application IHC-IF (paraffin)

Publication Kwon, G. et al. A novel pan-Nox inhibitor, APX-115, protects kidney injury in streptozotocin-induced diabetic mice: possible role of peroxisomal and mitochondrial biogenesis. Oncotarget. 8, 74217-74232 (2017).

Species mouse

Application IHC (paraffin)

Publication Haase, R. et al. A novel in vivo method to quantify slit diaphragm protein abundance in murine proteinuric kidney disease. PLoS One 12, e0179217 (2017)

Species mouse

Application WB

Publication Musah, S. et al. Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip. Nat.Biomed.Eng. 1,- (2017).

Species human

Application ICC-IF

Publication Königshausen, E. et al. Angiotensin II increases glomerular permeability by ?-arrestin mediated nephrin endocytosis. Nat. Publ. Gr. 6, (2016).

Species mouse

Application WB,IHC (paraffin),IP

Publication Sharmin, S. et al. Human Induced Pluripotent Stem Cell-Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation. J. Am. Soc. Nephrol. 27, 1778-1791 (2016).

Species human

Application IHC,ICC-IF,FACS

Publication Vasilopoulou, E. et al. Loss of endogenous thymosin ?4 accelerates glomerular disease. Kidney Int. 90, 1056-1070 (2016).

Species mouse

Application IHC (paraffin)

Publication Denhez, B. et al. Increased SHP-1 Protein Expression by High Glucose Levels Reduces Nephrin Phosphorylation in Podocytes. J. Biol. Chem. -290, 350-359 (2015).

Species mouse

Application IHC (paraffin)

Publication Schell, C. et al. Podocyte-Specific Deletion of Murine CXADR Does Not Impair Podocyte Development, Function or Stress Response. PLoS One 10, e0129424 (2015).

Species mouse

Application IHC (frozen)

Publication Gödel, M. et al. Microtubule Associated Protein 1b (MAP1B) Is a Marker of the Microtubular Cytoskeleton in Podocytes but Is Not Essential for the Function of the Kidney Filtration Barrier in Mice. PLoS One 10, (2015).

Species rat

Application IHC (frozen)

Publication Hyvönen, M. E. et al. Early-Onset Diabetic E1-DN Mice Develop Albuminuria and Glomerular Injury Typical of Diabetic Nephropathy. Biomed Res. Int. 2015, (2015).

Species mouse

Application IHC (frozen)

Publication Schulte, K. et al. Origin of parietal podocytes in atubular glomeruli mapped by lineage tracing. J. Am. Soc. Nephrol. 25, 129-41 (2014).

Species mouse

Application IHC (frozen)

Publication Ihara, K. I. et al. MAGI-2 is critical for the formation and maintenance of the glomerular filtration barrier in mouse kidney. Am. J. Pathol. 184, 2699-2708 (2014).

Species mouse

Application IHC (frozen),IEM

Publication Hartleben, B. et al. aPKC?/? and aPKC? contribute to podocyte differentiation and glomerular maturation. J. Am. Soc. Nephrol. 24, 253-67 (2013).

Species mouse

Application IHC (frozen)

Publication Bechtel, W. et al. Vps34 deficiency reveals the importance of endocytosis for podocyte homeostasis. J. Am. Soc. Nephrol. 24, 727-43 (2013).

Species mouse

Application IHC (paraffin)

Publication Boerries, M. et al. Molecular fingerprinting of the podocyte reveals novel gene and protein regulatory networks. Kidney Int. 83, 1052-1064 (2013).

Species mouse

Application WB

Publication Hartleben, B. et al. Role of the Polarity Protein Scribble for Podocyte Differentiation and Maintenance. PLoS One 7, (2012).

Species mouse

Application IHC (frozen)

Publication Niethamer, T. K. et al. Oral monosaccharide therapies to reverse renal and muscle hyposialylation in a mouse model of GNE myopathy. Mol. Genet. Metab. -107, 748-755 (2012).

Species mouse

Application WB

Publication Kakani, S. et al. The Gne M712T Mouse as a Model for Human Glomerulopathy. Am. J. Pathol. 180, 1431-1440 (2012).

Species mouse

Application WB

Publication Haege, S. et al. CXC Chemokine Receptor 7 (CXCR7) Regulates CXCR4 Protein Expression and Capillary Tuft Development in Mouse Kidney. PLoS One 7, (2012).

Species mouse

Application IHC (paraffin)

Publication Shimizu, A. et al. ARB protects podocytes from HIV-1 nephropathy independently of podocyte AT1. Nephrol. Dial. Transplant. 27, 3169-3175 (2012).

Species mouse

Application IHC (paraffin)

Publication Wagner, N. et al. The podocyte protein nephrin is required for cardiac vessel formation. Hum. Mol. Genet. 20, 2182-2194 (2011).

Species mouse

Application IHC (frozen),IHC (paraffin)

Publication Matsusaka, T. et al. Podocyte injury damages other podocytes. J. Am. Soc. Nephrol. 22, 1275-85 (2011).

Species mouse

Application IHC (paraffin)

Publication Matsusaka, T. et al. Glomerular sclerosis is prevented during urinary tract obstruction due to podocyte protection. Am. J. Physiol. - Ren. Physiol. 300, F792-800 (2011).

Species mouse

Application WB,IHC (paraffin)

Publication Inoki, K. et al. mTORC1 activation in podocytes is a critical step in the development of diabetic nephropathy in mice. J. Clin. Invest. 121, 2181-2196 (2011).

Species human

Application IHC (frozen)

Publication Meyer-Schwesinger, C. et al. Nephrotic Syndrome and Subepithelial Deposits in a Mouse Model of Immune-Mediated Anti-Podocyte Glomerulonephritis. J. Immunol. 187, 3218-3229 (2011).

Species mouse

Application IHC (paraffin)

Publication Gödel, M. et al. Role of mTOR in podocyte function and diabetic nephropathy in humans and mice. J. Clin. Invest. 121, 2197-2209 (2011).

Species mouse

Application IHC (frozen)

Publication Puliti, A. et al. Albuminuria and Glomerular Damage in Mice Lacking the Metabotropic Glutamate Receptor 1. Am. J. Pathol. 178, 1257-1269 (2011).

Species mouse

Application WB,IHC (frozen),ICC-IF

Publication Matsusaka, T. et al. ARB Protection Against Podocyte-Induced Sclerosis is Podocyte AT1-Independent. Hypertension 55, 967-973 (2010).

Species mouse

Application IHC (paraffin)

Publication Li, Y. et al. Inhibition of integrin-linked kinase attenuates renal interstitial fibrosis. J. Am. Soc. Nephrol. 20, 1907-18 (2009).

Species mouse

Application WB,IHC (frozen)

Publication Turk, T. et al. BMP Signaling and Podocyte Markers Are Decreased in Human Diabetic Nephropathy in Association With CTGF Overexpression. J. Histochem. Cytochem. 57, 623-631 (2009).

Species human

Application IHC (paraffin)

Publication Takabatake, Y. et al. The CXCL12 (SDF-1)/CXCR4 axis is essential for the development of renal vasculature. J. Am. Soc. Nephrol. 20, 1714-23 (2009).

Species mouse

Application IHC (frozen)

Publication Uchida, K. et al. Decreased tyrosine phosphorylation of nephrin in rat and human nephrosis. Kidney Int. 73, 926-932 (2008).

Species human

Application IHC

Publication Favaro, E. et al. Hyperglycemia Induces Apoptosis of Human Pancreatic Islet Endothelial Cells Effects of Pravastatin on the Akt Survival Pathway. Am. J. Pathol. 173, 442-450 (2008).

Species human

Application WB,IP

Publication Collino, F. et al. Preeclamptic sera induce nephrin shedding from podocytes through endothelin-1 release by endothelial glomerular cells. Am. J. Physiol. - Ren. Physiol. 294, 1185-1194 (2008).

Species human

Application WB,ICC-IF

Publication Wagner, K.-D. et al. Report An Inducible Mouse Model for PAX2-Dependent Glomerular Disease: Insights into a Complex Pathogenesis. Curr. Biol. 16, 793-800 (2006).

Species human

Application IHC

Publication Bussolati, B. et al. Statins Prevent Oxidized LDL-Induced Injury of Glomerular Podocytes by Activating the Phosphatidylinositol 3-Kinase/ AKT-Signaling Pathway. J. Am. Soc. Nephrol.- JASN -16, 1936-1947 (2005).

Species human

Application ICC-IF

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File IHC guinea pig antibody

Category Protocol

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Datasheet

MSDS

FAQs

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

Kidney frozen in OCT
5 µm sections
Slides fixed 5 min in 100% acetone (precooled to -20°C) and kept at -80°C
Blocking with 2% FCS or 2% BSA and 0.2% fish gelatine in PBS (as alternative blocking with 2% mouse serum can be performed to reduce background)
Incubation with GP-N2 in blocking solution for 1h in moist chamber at RT (optimal dilution needs to be tritrated)
Wash 3x with PBS
Incubation with fluorescent secondary anti-guinea pig antibody diluted in blocking solution for 1h in moist chamber at RT
Wash 3x with PBS
Mounting

The expiration date of our antibodies is indicated on the product label.

When using podocyte cell lines, the cells needs to be 90% confluent, since nephrin is only expressed if cell-cell contacts are present.
When using kidney tissue, it is often important to concentrate the glomeruli.
RIPA buffer is recommended for protein extraction.

Methanol/ acetone fixation: Immerse slide in precooled (-20°C) methanol for 5 min, immerse in precooled (-20°C) acetone for 30-60 sec, let specimen air dry before antibody incubation.
Methanol/ acetone fixation plus detergent permeabilization: After methanol/ acetone fixation and air-drying dip slide either in a solution containing 0.1-0.2% Triton X-100 in PBS or in 0.1% saponin in PBS for 1-5 min at room temperature (enhances accessibility of many cytoskeletal antigens).

Air-drying of the section.
Block with the serum of the species in which the secondary antibody was raised for 30 min.
Incubation with 1st antibody 1 h at RT in moist chamber.
Wash 3x with PBS.
Incubation with appropriate fluorescent secondary antibody, 30-60 min at RT.
Wash 3x with PBS.
Immerse shortly into ethanol.
Let air dry.
Cover with mounting medium.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.

We recommend a heat reduced antigen retrieval protocol with citrate buffer pH 6.0.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.

PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

The concentration of specific antibody in our guinea pig serum is not determined.
In guinea pigs the antibody concentration in serum varies from 10 to 20 mg/ml; specific antibodies represent normally about 0.1-1% of total IgG. Total protein concentration varies from 40 to 65 mg/ml, with the main constituent (about 60%) being albumin.

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anti-Nephrin guinea pig polyclonal, serum

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Which IHC protocol is recommended for staining of frozen sections with anti-nephrin, Cat. No. GP-N2?

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Tips for WB with anti-nephrin, Cat. No. GP-N2

Protocol for frozen sections with guinea pig antibodies

Do the PROGEN antibodies contain a preservative?

Which antigen retrieval is recommended for anti-nephrin, Cat. No. GP-N2?

How can I find the optimal antibody dilution for my experiment?

How are PROGEN antibodies shipped?

What is the concentration of specific antibody in guinea pig serum?