Western blot with anti-Nucleolar Helicase on various tissues (courtesy of The Human Protein Atlas, www.proteinatlas.org, Thul PJ et al, 2017. A subcellular map of the human proteome. Science)
Western blot with anti-Nucleolar Helicase on various tissues (courtesy of The Human Protein Atlas, www.proteinatlas.org, Thul PJ et al, 2017. A subcellular map of the human proteome. Science)
Key Features
- Mouse monoclonal
- Suitable for IHC and WB
- Reacts with human
- Isotype: IgG1
Product description
| Quantity | 250 µl |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Application | IHC, WB |
| Purification | Hybridoma cell culture supernatant concentrate |
| Reactivity | Human |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | No-440 |
| Immunogen | Human recombinant polypeptide |
| Formulation | Contains 0.09% sodium azide |
| UniprotID | |
| Synonym | ATP-dependent 61 kDa nucleolar RNA helicase, DEAD (Asp-Glu-Ala-Asp) box helicase 56, DEAD (Asp-Glu-Ala-Asp) box polypeptide 56, DEAD box protein 21, DEAD box protein 56, DEAD-box RNA helicase, Probable ATP-dependent RNA helicase DDX56 |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:500–1:1,000 |
| Western Blot (WB) | 1:3,000-1:5,000 |
Background
The antibody recognizes the nucleolar helicase protein NOH61 (61 kDa), a constitutive nucleolar protein which has been highly conserved during evolution.
NO-440 stains specifically the granular component of the nucleolus in interphase cells. During mitosis the protein is localized accumulated around the condensed chromosomes.
FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.