anti-Nucleolar Helicase mouse monoclonal, No-440, supernatant concentrate
- Mouse monoclonal
- Suitable for IHC and WB
- Reacts with human
- Isotype: IgG1
|Purification||Hybridoma cell culture supernatant concentrate|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Human recombinant polypeptide|
|Formulation||Contains 0.09% sodium azide|
|Synonym||ATP-dependent 61 kDa nucleolar RNA helicase, DEAD (Asp-Glu-Ala-Asp) box helicase 56, DEAD (Asp-Glu-Ala-Asp) box polypeptide 56, DEAD box protein 21, DEAD box protein 56, DEAD-box RNA helicase, Probable ATP-dependent RNA helicase DDX56|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:500–1:1,000|
|Western Blot (WB)||1:3,000-1:5,000|
The antibody recognizes the nucleolar helicase protein NOH61 (61 kDa), a constitutive nucleolar protein which has been highly conserved during evolution.
NO-440 stains specifically the granular component of the nucleolus in interphase cells. During mitosis the protein is localized accumulated around the condensed chromosomes.
Q & A's
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The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.