Disease related

anti-Nucleolar Protein NO38 mouse monoclonal, No-185, supernatant concentrate

Cat. No. 652125

Available, delivery time 1-7 days


Key Features
  • Mouse monoclonal
  • Suitable for IHC, IP and WB
  • Reacts with chicken, pleurodelis, triturus and xenopus
  • Isotype: IgG1
Product description
Quantity 250 µl
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, IP, WB
Purification Hybridoma cell culture supernatant concentrate
Reactivity Chicken, Pleurodeles, Triturus, Xenopus
No reactivity Higher vertebrates
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone No-185
Immunogen Nucleolar fraction prepared from Xenopus laevis oocytes
Formulation Contains 0.09% sodium azide
Synonym Nucleophosmin, NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin
Note Centrifuge prior to opening
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:300-1:500 (fixation with paraformaldehyde recommended)
Immunoprecipitation (IP) Assay dependent
Western Blot (WB) 1:1,000-1:2,000

The antibody recognizes a 38 kDa nucleolar protein (NO38) in Xenopus (this protein has also been termed B23, nucleophosmin, numatrin in other species).
No-185 stains specifically the granular component of the nucleolus and small nucleolar dots in interphase cells. During mitosis the protein is localized accumulated around the condensed chromosomes.
The epitope recognized is different from the one recognized by clone No-63 (cat.no. 612123).

References/Publications (4)
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The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

Related Products

anti-Nucleolar Protein NO38 mouse monoclonal, No-63, ascites fluid
Cat. No. 612123
Quantity: 250 µl
  • Ascites fluid
  • Mouse monoclonal
  • Suitable for IHC, IP and WB
  • Reacts with chicken, pleurodelis, triturus and xenopus
  • Isotype: IgG2a

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