Disease related

anti-Nucleolar Protein NO38 mouse monoclonal, No-185, supernatant concentrate

Cat. No. 652125

Available, delivery time 1-7 days

€279.00*

Key Features
  • Mouse monoclonal
  • Suitable for IHC, IP and WB
  • Reacts with chicken, pleurodelis, triturus and xenopus
  • Isotype: IgG1
Product description
Quantity 250 µl
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, IP, WB
Purification Hybridoma cell culture supernatant concentrate
Reactivity Chicken, Pleurodeles, Triturus, Xenopus
No reactivity Higher vertebrates
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone No-185
Immunogen Nucleolar fraction prepared from Xenopus laevis oocytes
Formulation Contains 0.09% sodium azide
UniprotID
Synonym Nucleophosmin, NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:300-1:500 (fixation with paraformaldehyde recommended)
Immunoprecipitation (IP) Assay dependent
Western Blot (WB) 1:1,000-1:2,000
Background

The antibody recognizes a 38 kDa nucleolar protein (NO38) in Xenopus (this protein has also been termed B23, nucleophosmin, numatrin in other species).
No-185 stains specifically the granular component of the nucleolus and small nucleolar dots in interphase cells. During mitosis the protein is localized accumulated around the condensed chromosomes.
The epitope recognized is different from the one recognized by clone No-63 (cat.no. 612123).

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