Disease related

anti-Nucleolus-Organizing Region mouse monoclonal, X-11 + X-19, supernatant

Cat. No. 65148

Available, delivery time 1-7 days

€143.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Isotype IgG1
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Xenopus
No reactivity Chicken, Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone X-11 + X-19
Immunogen Nuclear pore complex-lamina fraction of XLKE-A6 cells (cultured cell line derived from Xenopus laevis)
Formulation Contains 0.09% sodium azide
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen Ready-to-use
Immunohistochemistry (IHC) - paraffin Ready-to-use (microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

The mabs represent marker for nucleolar organizer region (NOR), putatively staining the protein treacle (described as TCOF1).

Polypeptides reacting: Mr 240 kDa band in SDS gels and lower molecular weight bands (e.g. 160 kDa band, Gonzales et al. 2005).

Gonzales B, Yang H, Henning D, Valdez BC: Cloning and functional characterization of the Xenopus orthologue of the Treacher Collins syndrome (TCOF1) gene product. Gene 359, 73-80 (2005)

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