Key Features
- Mouse monoclonal
- Suitable for ICC/IF, IHC, IP and WB
- Reacts with human
- Isotype: IgM
Product description
| Quantity | 5 ml |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgM |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC, IP, WB |
| Purification | Hybridoma cell culture supernatant |
| Reactivity | Human |
| Storage | 2-8°C |
| Intended use | Research use only |
| Clone | mESL |
| Immunogen | Synthetic C-terminus (21 aa) of human organic anion transporter SLC21A6 coupled to KLH |
| Formulation | Contains 0.09% sodium azide |
| UniprotID | Q9NPD5 (Human) |
| Synonym | Solute carrier organic anion transporter family member 1B3, Liver-specific organic anion transporter 2, LST-2, Organic anion transporter 8, Organic anion-transporting polypeptide 8, OATP-8, Solute carrier family 21 member 8, SLCO1B3, LST2, OATP1B3, OATP8, SLC21A8 |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | Assay dependent |
| Immunohistochemistry (IHC) - frozen | Ready-to-use |
| Immunohistochemistry (IHC) - paraffin | Ready-to-use (microwave treatment recommended) |
| Immunoprecipitation (IP) | Assay dependent |
| Western Blot (WB) | Assay dependent (for optimal detection of the glycosylated antigen sample boiling before SDS-PAGE is not recommended; alternatively, samples should be kept at 37°C for 30 min) |
Background
In Western blot analyses the monoclonal antibody mESL reacts selectively with the 90 kDa protein (after SDS-PAGE) present in the basolateral membrane fractions of human liver. According to a recent new nomenclature this hepatocyte-specific protein is identical to OATP1B1 (Hagenbuch and Meier, 2003) also known as OATP-C.
Using immunofluorescence microscopy mESL stains the sinusoidal (basolateral) membranes in frozen sections of human liver, hepatocellular carcinoma and the human cell line HEK293 transfected with SLC21A6.
Polypeptide reacting: OATP2/OATP1B1, human organic anion transporter SLC21A6 (90 kDa).
Reactivity on cultured cell lines: SCL21A6 transfected HEK293 cells; negative with HepG2 and PLC.
Hagenbuch E, Meier PJ: The superfamily of organic anion transporting polypeptides. Biochim Biophys Acta 1609, 1-18 (2003)
References/Publications (1)
Publication
Species
Application
Species
human
Application
WB,IHC,ICC-IF
FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
- The antibody is an IgM subtype with low affinity, therefore the dilution should not be too high.
- The primary antibody should be incubated overnight at 2-8°C.
- The sample in SDS-buffer should only be heated up to 37°C for 30 min, do not boil the sample.
- The use of fresh (undegraded) membrane preparations is recommended.
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
PROGEN offers different formats for mouse monoclonal antibodies. Different antibodies may not be offered in each format, the following are all possible formats:
- Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
- Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
- Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
- Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.
The recommended temperature during antigen retrieval for IHC is 60°C. Samples should not be boiled (100°C) because boiling may affect detection of highly glycosylated proteins.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
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- Mouse monoclonal
- Suitable for ICC/IF, IHC, IP and WB
- Reacts with human
- Isotype: IgG1