Frequently used

anti-Perilipin 1 (C-terminus) guinea pig polyclonal, serum

Cat. No. GP33

Available, delivery time 1-7 days

€279.00*

Key Features
  • Guinea pig polyclonal
  • Suitable for IHC and WB
  • Reacts with human, mouse and rat
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application IHC, WB
Purification Stabilized antiserum
Reactivity Human, Mouse, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen C-terminus of human perilipin / PLIN1 (hCTA/B; aa 507 - 519; cf. Greenberg et al. 1992, JBC 266, 11341-11346)
Formulation Contains 0.09% sodium azide and 0.5% BSA
UniprotID O60240 (Human),Q8CGN5 (Mouse),P43884 (Rat)
Synonym Perilipin-1, Lipid droplet-associated protein, PLIN1, PERI, PLIN
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:100-1:200
Immunohistochemistry (IHC) - paraffin 1:100-1:200 (microwave treatment recommended)
Western Blot (WB) 1:1,000-1:2,000
Background

Perilipins build a family of phosphoproteins. The predominant forms in adipocytes, perilipin / PLIN1 A and B arise by alternative RNA splicing from a single gene, generating polypeptides of 57 and 46 kD, respectively.
The antiserum reacts specifically with perilipin / PLIN1 located at the surface of intracellular storage lipid droplets present e.g. in the adrenal gland, adipocytes of white and brown adipose tissue and cultured cells such as adipocytes and cultured steroidogenic adrenal cortical and Leydig cells. The antiserum does not cross-react with adipophilin (ADRP, also named PLIN2) or TIP47 (also named PLIN3) proteins, or additional members of the PLIN/PAT-family , e.g. MLDP or OXPAT/PAT-1, also named PLIN5, LSDP5.


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