anti-Perilipin 2 (N-terminus aa 1-29) guinea pig polyclonal, serum, sample (ready-to-use)
- Stabilized antiserum, ready-to-use for IHC
- Guinea pig polyclonal
- Suitable for ICC/IF, IHC and WB
- Reacts with human
|Quantity||600 µl (ready-to-use)|
|Application||ICC/IF, IHC, WB|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Synthetic peptide (N-terminal aa 1-29 of human and murine adipophilin)|
|Formulation||Contains 0.09% sodium azide and 0.5% BSA|
|UniprotID||Q99541 (Human),P43883 (Mouse)|
|Synonym||Perilipin-2, Adipophilin, Adipose differentiation-related protein, ADRP, PLIN2, ADFP|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (IHC) - paraffin||Ready-to-use (microwave treatment recommended)|
|Western Blot (WB)||Assay dependent|
Perilipin 2/Adipophilin/ADRP/PLIN2 (a member of the PLIN/PAT family) is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines; inducible by etomoxir.
Enhanced expression of Perilipin 2/Adipophilin/ADRP/PLIN2 is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis.
ADRP can also be used to study the virus entry via lipid droplets.
Polypeptide reacting: specific for Perilipin 2/Adipophilin/ADRP/PLIN2, MW 48,100 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE); pI 6.72.
Immunolocalization: Adipophilin/PLIN2 is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative.
Also positively stained are lipid-storing CD 68-positive macrophages.
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- Purified, lyophilized
- Mouse monoclonal
- Suitable for IHC and WB
- Reacts with dog, human and rat
- Isotype: IgG1