Frequently used

anti-Perilipin 2 (N-terminus aa 1-29) guinea pig polyclonal, serum, sample (ready-to-use)

Serum

Sample

Cat. No. GP40S

Available, delivery time 1-7 days

€49.00*

Key Features
  •  Stabilized antiserum, ready-to-use for IHC
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human
Product description
Quantity 600 µl (ready-to-use)
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Stabilized antiserum
Reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Synthetic peptide (N-terminal aa 1-29 of human and murine adipophilin)
Formulation Contains 0.09% sodium azide and 0.5% BSA
UniprotID Q99541 (Human),P43883 (Mouse)
Synonym Perilipin-2, Adipophilin, Adipose differentiation-related protein, ADRP, PLIN2, ADFP
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - paraffin Ready-to-use (microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

Perilipin 2/Adipophilin/ADRP/PLIN2 (a member of the PLIN/PAT family) is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines; inducible by etomoxir.
Enhanced expression of Perilipin 2/Adipophilin/ADRP/PLIN2 is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis.
ADRP can also be used to study the virus entry via lipid droplets.
Polypeptide reacting: specific for Perilipin 2/Adipophilin/ADRP/PLIN2, MW 48,100 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE); pI 6.72.
Immunolocalization: Adipophilin/PLIN2 is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative.
Also positively stained are lipid-storing CD 68-positive macrophages.


References/Publications (39)
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Category Protocol
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Category Protocol
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