anti-Perilipin 2 (mouse N-terminus) guinea pig polyclonal, serum

Cat. No. GP42

Available, delivery time 1-7 days

€299.00*

Key Features
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with mouse
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Stabilized antiserum
Reactivity Mouse
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Synthetic peptide (N-terminal aa 1-16 of murine adipophilin, also named PLIN2): MAAAVVDPQQSVVMRV-C
Formulation Contains 0.09% sodium azide and 0.5% BSA
UniprotID Q99541 (Human),P43883 (Mouse)
Synonym Perilipin-2, Adipophilin, Adipose differentiation-related protein, ADRP, PLIN2, ADFP
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:50-1:100
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (microwave treatment recommended)
Western Blot (WB) 1:500-1:3,000
Background

Adipophilin / ADRP / PLIN2 (a member of the PLIN/PAT family) is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (see e.g. Heid et al.1998, 2013; Chong et al.).
Polypeptide reacting: Specific for Adipophilin / ADRP / PLIN2, MW 46,646 (calculated from aa sequence data); apparent Mr 50,000 (after SDS-PAGE); pI 6.42.
Tissue immunolocalization: Adipophilin / PLIN2 is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes; adipocytes are negative.
Reactivity on cultured cell lines: 3T3-L1, OP9, HL-1


Heid, H. et al. Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells. PLoS One 8, (2013).
Chong, B. M. et al. The adipophilin C terminus is a self-folding membrane-binding domain that is important for milk lipid secretion. J. Biol. Chem. 286, 23254-23265 (2011). Heid, H. W., Moll, R., Schwetlick, I., Rackwitz, H. R. & Keenan, T. W. Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases. Cell Tissue Res. 294, 309-21 (1998).

References/Publications (1)
Publication
Species
Application
Downloads
File
Category
Size
Filetype
Category Protocol
Size 917.17 KB
Filetype pdf
Q & A's

Please enter a valid email address.
Please enter a valid question.
Fields marked with * are mandatory.

There aren't any asked questions yet.
Customer Reviews

0 of 0 reviews

Leave a review!

Share your experiences with other customers.


Related Products

anti-guinea pig IgG goat polyclonal, HRP conjugate
Cat. No. 90001
  • Goat polyclonal
  • Suitable for ELISA and WB
  • Reacts with guinea pig

€90.00*
anti-Perilipin 1,2,5 sample set
Cat. No. 70012
The anti-Perilipin 1,2,5 sample set provides antibodies directed against Perilipin 1, 2 and 5 to evaluate the presence and status in IHC and WB.

€110.00*
anti-Perilipin 2,3,5 sample set
Cat. No. 70011
The anti-Perilipin 2,3,5 sample set provides antibodies directed against Perilipin 2, 3 and 5 to evaluate the presence and status in IHC and WB.

€110.00*
anti-Perilipin 1-5 complete sample set
Cat. No. 70010
The anti-Perilipin 1-5 complete sample set provides antibodies directed against all 5 Perilipins to evaluate the presence and status in IHC and WB.

€190.00*
anti-Perilipin 2 (N-terminus) mouse monoclonal, AP125, lyophilized, purified
Cat. No. 610102
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with dog, human and rat
  • Isotype: IgG1

Variants from €49.00*
€294.00*
anti-Perilipin 2 (N-terminus aa 6-27) guinea pig polyclonal, serum
Cat. No. GP47
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human and mouse

€299.00*
anti-Perilipin 2 (N-terminus aa 1-16) guinea pig polyclonal, serum
Cat. No. GP46
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human and mouse

€299.00*
anti-Perilipin 2 (C-terminus) guinea pig polyclonal, serum
Cat. No. GP41
  • Guinea pig polyclonal
  • Suitable for IHC and WB
  • Reacts with human

€299.00*
anti-Perilipin 2 (N-terminus aa 1-29) guinea pig polyclonal, serum
Cat. No. GP40
Quantity: 100 µl
  • Stabilized antiserum
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human

Variants from €49.00*
€299.00*