anti-Perilipin 4 (C-terminus) guinea pig polyclonal, serum, sample (ready-to-use)
- Stabilized antiserum, ready-to-use for IHC
- Guinea pig polyclonal
- Suitable for ICC/IF, IHC and WB
- Reacts with human
|Quantity||600 µl (ready-to-use)|
|Application||ICC/IF, IHC, WB|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Synthetic peptide (C-terminal aa 1394-1410 of human S3-12 (also named PLIN4); C-QGLEQLLEGLQHNPPLS, coupled to KLH)|
|Formulation||Contains 0.09% sodium azide and 0.5% BSA|
|Synonym||Perilipin-4, Adipocyte protein S3-12, PLIN4, KIAA1881|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (IHC) - frozen||Ready-to-use|
|Immunohistochemistry (IHC) - paraffin||Ready-to-use (microwave treatment recommended)|
|Western Blot (WB)||Assay dependent|
Polypeptide reacting: S3-12, apparent Mr 140,000 (after SDS-PAGE).
S3-12 pertains to the PLIN/PAT-family proteins, covering the surface of cytoplasmic lipid droplets.
Additional PAT proteins include adipophilin (ADRP/PLIN2), perilipin (PLIN1), TIP47 (PLIN3), and MLDP (OXPAT/PAT-1, PLIN5 or LSDP5) which are expressed in differentiation-related stages of lipid metabolism.
No cross-reactivity with additional PLIN/PAT proteins (including adipophilin/ADRP/PLIN2, perilipin/PLIN1, and TIP47/PLIN3) which are expressed in differentiation-related stages of lipid metabolism.
Tissue Immunolocalization: S3-12/PLIN4 is positively detected in lipid droplets of cultured cells.
Reactivity on cultured cell lines: PLC (human).
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