anti-Plakophilin 2 guinea pig polyclonal, serum

Cat. No. GP-PP2

Available, delivery time 1-7 days

€281.00*

Key Features
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human, mouse, pig and rat
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Stabilized antiserum
Reactivity Bovine, Human, Mouse, Pig, Rat
No reactivity Amphibia, Fish
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Human synthetic plakophilin 2 (C-terminal aa 820-8 37)
Formulation Contains 0.09% sodium azide and 0.5% BSA
UniprotID A0A3Q1M2G3 (Bovine), Q99959 (Human), Q9CQ73 (Mouse), A0A4X1VWB0 (Pig), H0VL79 (Guinea pig), Q562C0 (Rat)
Synonym Plakophilin-2, PKP2
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:100 (preincubated for 5 min with 0.2% Triton X-100 prior to antibody application recommended; longer incubation could result in a loss of nuclear PP2-staining; fixation with 2% formaldehyde to prevent wash-out of soluble forms)
Immunohistochemistry (IHC) - paraffin 1:100 (microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

PP2 antiserum stains specifically desmosomal plaques present in simple and glandular epithelia, the basal layer of certain stratified epithelia, all layers of some stratified epithelia, and desmosome-possessing nonepithelial cells such as myocardium, Purkinje fibers, and dendritic reticulum of lymph node follicles. In all these cells, including a variety of cell types devoid of desmosomes, plakophilin 2 is highly enriched in the karyoplasm.
Polypeptide reacting: plakophilin 2, Mr ~100 kDa (SDS-PAGE); MW calculated from aa sequence: 92,750 (pI 9.33) and 97,410 (pI 9.38).

Reactivity on cultured cell lines: MCF-7, PLC.

References/Publications (3)
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