anti-RAP rabbit polyclonal, serum
- Rabbit polyclonal
- Suitable for IHC, receptor binding assay and WB
- Reacts with chicken, human and rat
|Application||IHC, Receptor binding assay, WB|
|Reactivity||Chicken, Human, Rat|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Recombinant human RAP (receptor-associated protein)|
|Formulation||Contains 0.09% sodium azide and 0.5% BSA|
|UniprotID||P62834 (Human),P62836 (Rat)|
|Synonym||Ras-related protein Rap-1A, EC 126.96.36.199, C21KG, G-22K, GTP-binding protein smg p21A, Ras-related protein Krev-1, RAP1A, KREV1|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:50–1:100|
|Immunohistochemistry (IHC) - paraffin||1:50–1:100 (microwave treatment recommended)|
|Receptor Binding Assays||Assay dependent|
|Western Blot (WB)||Assay dependent|
The antibody binds to an epitope of the C-terminal part of RAP, a sequence common to the human VLDL receptor superfamily (glycoprotein 330 and LRP), the chicken oocyte receptor for VLDL and vitellogenin (LR8), and glycoprotein 330/megalin from rat. No reaction with LDL receptor. The antibody interferes with binding of VLDL and vitellogenin receptor. Binding is Ca2+-dependent with LR8.
Antigen recognized in species:
|human||RAP, CD91 (LRP), VLDL-R|
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.