Key Features
- Rabbit polyclonal
- Suitable for IHC, receptor binding assay and WB
- Reacts with chicken, human and rat
Product not available in China
Product description
| Quantity | 250 µl |
|---|---|
| Antibody Type | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Application | IHC, Receptor binding assay, WB |
| Purification | Stabilized antiserum |
| Reactivity | Chicken, Human, Rat |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Immunogen | Recombinant human RAP (receptor-associated protein) |
| Formulation | Contains 0.09% sodium azide and 0.5% BSA |
| UniprotID | P62834 (Human),P62836 (Rat) |
| Synonym | Ras-related protein Rap-1A, EC 3.6.5.2, C21KG, G-22K, GTP-binding protein smg p21A, Ras-related protein Krev-1, RAP1A, KREV1 |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:50–1:100 |
| Immunohistochemistry (IHC) - paraffin | 1:50–1:100 (microwave treatment recommended) |
| Receptor Binding Assays | Assay dependent |
| Western Blot (WB) | Assay dependent |
Background
The antibody binds to an epitope of the C-terminal part of RAP, a sequence common to the human VLDL receptor superfamily (glycoprotein 330 and LRP), the chicken oocyte receptor for VLDL and vitellogenin (LR8), and glycoprotein 330/megalin from rat. No reaction with LDL receptor. The antibody interferes with binding of VLDL and vitellogenin receptor. Binding is Ca2+-dependent with LR8.
Antigen recognized in species:
| human | RAP, CD91 (LRP), VLDL-R |
| chicken | LR8 |
| rat | gp330 |
FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
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anti-RAP mouse monoclonal, 7F1, lyophilized w/o sodium azide, purified
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Cat. No. 61068
- Mouse monoclonal
- Suitable for IHC, receptor binding assays and WB
- Reacts with human
- Isotype: IgG1