anti-RNA Polymerase II mouse monoclonal, ARNA-3, supernatant concentrate
- Mouse monoclonal
- Suitable for ICC/IF, IHC and WB
- Reacts with drosophila and human
- Isotype: IgG1
|Application||ICC/IF, IHC, WB|
|Purification||Hybridoma cell culture supernatant concentrate|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Purified drosophila melanogaster RNA polymerase II|
|Formulation||Contains 0.09% sodium azide|
|Synonym||DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, EC 18.104.22.168, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, EC 22.214.171.124, POLR2A, POLR2|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||Assay dependent (recommended fixation conditions for cells: incubate in 2% paraformaldehyde in PBS for 10 min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min)|
|Immunohistochemistry (IHC) - frozen||1:100|
|Western Blot (WB)||Assay dependent|
ARNA-3 is excellent for detection of transcription activity, e.g. puffs in polytene chromosomes. Reacts with RNA polymerase II (Mr 175,000 subunit of RNAP II) in biological materials. Reactive polypeptides after SDS-PAGE: 200 kD band of nonphosphorylated and 240 kD band of phosphorylated Pol IIA.
Epitope recognized within: 794-DDYGPESRGFVENSYLA-822
Q & A's
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The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
- Use wet transfer instead of semidry method.
- Reduce the amount of methanol in the transfer buffer (< 20%).
- Increase the transfer time (e.g. on at 100-200 mA).
- If the protein is low abundant (e.g. nephrin expressed only in podocytes) enrich your protein of interest.
- Pretreatment of the sample with DNase.