anti-Symplekin mouse monoclonal, Sym-TJ-E150, supernatant
Species/Reactant: Homo sapiens (Human)
Applications: Western blotting following immunoprecipitation
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- Mouse monoclonal
- Suitable for IHC and WB
- Reacts with human and rat
- Isotype: IgG1
|Purification||Hybridoma cell culture supernatant|
|No reactivity||Bovine, Mouse, Xenopus|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Formulation||Contains 0.09% sodium azide|
|UniprotID||Q92797 (Human), F1LSH0 (Rat)|
|Synonym||Symplekin, SYMPK, SPK|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||Ready-to-use (for nucleoplasmic staining a modified Carnoy fixation procedure is suggested (60% ethanol, 30% chloroform, 10% acetic acid) for 20 min, followed by a 10 min ethanol wash)|
|Immunohistochemistry (IHC) - paraffin||Ready-to-use (microwave treatment recommended; for nucleoplasmic staining a modified Carnoy fixation procedure is suggested (60% ethanol, 30% chloroform, 10% acetic acid) for 20 min, followed by a 10 min ethanol wash)|
|Western Blot (WB)||Assay dependent|
The mab reacts specifically with symplekin (a 150 kDa polypeptide) present in 1) tight junctions (zonula occludens) of polar epithelia and Sertoli cells of testis (but not their vascular endothelia!) and 2) exclusively in the nucleoplasm of these and many other cell types which do not form tight junctions (e.g. stratified epithelia such as oesophagus, tongue mucosa, epidermis; squamous cell carcinoma; non-epithelia such as fibroblasts, endothelia, lymphocytes).
Polypeptide reacting: symplekin, 126.5 kDa polypeptide (calculated from aa sequence data); apparent Mr 150 kDa (after SDS-PAGE); pI 6.5.
Reactivities on cultured cell lines: carcinoma cell lines (e.g. MCF-7, Caco-2, HT29, PLC), SV80 (fibroblasts), U333 CG/343 MG (glioma), K 562 (erythroleukemia), RVF (rat vascular smooth muscle cells).
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
- Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
- Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
- Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
- Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.