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anti-Synaptophysin mouse monoclonal, SY38, prediluted, purified, ready-to-use

Mouse brain (courtesy of J. Heß, University Hospital Heidelberg)

Liquid

Liquid

Lyophilized

Prediluted

Cat. No. 65012

Prices excl. VAT

Available, delivery time: 1-7 days

€143.00*

Datasheet
Key Features
  • Purified, prediluted, ready-to-use for IHC
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human, mouse and rat
  • Isotype: IgG1
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Human, Mouse, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone SY38
Immunogen Synaptophysin from presynaptic vesicles, prepared from bovine brain
Formulation PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide
UniprotID P20488 (Bovine), P08247 (Human), Q62277 (Mouse), P07825 (Rat)
Synonym Synaptophysin, Major synaptic vesicle protein p38, SYP
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen Ready-to-use (no protease treatment)
Immunohistochemistry (IHC) - paraffin Ready-to-use (microwave treatment recommended, no protease treatment)
Western Blot (WB) Assay dependent
Background

SY38 represents an excellent marker for several neuroendocrine, neuronal and adrenal tumors. Neuronal and adrenal tumors such as pheochromocytomas, paragangliomas, neuroblastomas, ganglioneuroblastomas. Neuroendocrine tumors of epithelial origin: Pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids, medullary carcinoma of thyroid. Polypeptide reacting: 38 kDa transmembrane glycoprotein of presynaptic vesicles.

SY38 binds to a cytoplasmatic domain of synaptophysin. The epitope was located to a flexible segment in the center of the repeat structure (Knaus and Betz, 1990).

Tested cultured cell lines: rat PC-12 cell line.

Knaus, P. & Betz, H. Mapping of a dominant immunogenic region of synaptophysin, a major membrane protein of synaptic vesicles. FEBS Lett. 261, 358–360 (1990).

References/Publications (18)
Publication
Species
Application
Publication Matsuda, T and Oinuma, . Imaging endogenous synaptic proteins in primary neurons at single-cell resolution using CRISPR/Cas9. Mol.Biol.Cell. 30, 2838-2855 (2019)
Species mouse
Application ICC-IF
Publication Noble, E. et al. Control of Feeding Behavior by Cerebral Ventricular Volume Transmission of Melanin-Concentrating Hormone. Cell.Metab. 28, 55-68.e7 (2018)
Species rat
Application IHC-IF (paraffin)
Publication Hsu, T. M. et al. Hippocampus ghrelin signaling mediates appetite through lateral hypothalamic orexin pathways. Elife 4, (2016).
Species rat
Application IHC (frozen)
Publication Nakajima, C. et al. Low Density Lipoprotein Receptor-related Protein 1 (LRP1) Modulates N-Methyl-D-aspartate (NMDA) Receptor-dependent Intracellular Signaling and NMDA-induced Regulation of Postsynaptic Protein Complexes. J. Biol. Chem. 288, 21909-21923
Species mouse
Application WB,ICC-IF
Publication Sato, J., Sasaki, S., Yamada, N. & Tsuchitani, M. Hereditary Cerebellar Degenerative Disease (Cerebellar Cortical Abiotrophy) in Rabbits. Vet. Pathol. 49, 621-628 (2012).
Species rabbit
Application IHC (paraffin)
Publication Eispert, A.-C. et al. Evidence for distinct populations of human Merkel cells. Histochem. Cell Biol. 132, 83-93 (2009).
Species human
Application IHC (frozen)
Publication Matsuoka, H. et al. Molecular mechanisms supporting a paracrine role of GABA in rat adrenal medullary cells. J. Physiol. 58620, 4825-4842 (2008).
Species rat
Application ICC-IF
Publication Matsuoka, Y. et al. Impaired adrenocorticotropic hormone response to bacterial endotoxin in mice deficient in prostaglandin E receptor EP1 and EP3 subtypes. Proc. Natl. Acad. Sci. United States Am. -100, 4132-4137 (2003).
Species rat
Application IHC (frozen)
Publication Portela-Gomes, G. M., Stridsberg, M., Johansson, H. & Grimelius, L. Co-localization of synaptophysin with different neuroendocrine hormones in the human gastrointestinal tract. Histochem. Cell Biol. 111, 49-54 (1999).
Species human
Application IHC (paraffin)
Publication Chitaev, N. A. et al. The Binding of Plakoglobin to Desmosomal Cadherins: Patterns of Binding Sites and Topogenic Potential. J. Cell Biol. 113, 359-369 (1996).
Species human
Application WB
Publication Leube, R. E. The topogenic fate of the polytopic transmembrane proteins, synaptophysin and connexin, is determined by their membrane-spanning domains. J. Cell Sci. 108, 883-894 (1995).
Species human,rat
Application WB,ICC-IF
Publication Bennett, M. K., Calakos, N., Kreiner, T. & Scheller, R. H. Synaptic Vesicle Membrane Proteins Interact to Form a Multimeric Complex. J. Cell Biol. 116, 761-775 (1992).
Species rat
Application WB
Publication Knaus, P. & Betz, H. Mapping of a dominant immunogenic region of synaptophysin, a major membrane protein of synaptic vesicles. FEBS Lett. 261, 358–360 (1990).
Application WB, epitope mapping
Publication Dockhorn-Dworniczak, b. et al. patterns of expression of cytoskeletal proteins in human thyroid gland and thyroid carcinomas. differentiation. 35, 53-71 (1987).
Species human
Application IHC (frozen)
Publication Gould, V. E. et al. Synaptophysin Expression in Neuroendocrine Neoplasms as Determined by Immunocytochemistry. Am. J. Pathol. -126, 243-257 (1987).
Species human
Application IHC (frozen)
Publication Wiedenmann, B., Franke, W. W., Kuhn, C., Moll, R. & Gould, V. E. Synaptophysin: a marker protein for neuroendocrine cells and neoplasms. Proc. Natl. Acad. Sci. U. S. A. 83, 3500-4 (1986).
Species human
Application IHC (frozen)
Publication Gould, V. E. et al. Synaptophysin: a novel marker for neurons, certain neuroendocrine cells, and their neoplasms. Hum. Pathol. 17, 979-83 (1986).
Species human
Application IHC (paraffin)
Publication Wiedenmann, B. & Franke, W. W. Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles. Cell 41, 1017-28 (1985).
Species mouse
Application WB,IHC (frozen)
Downloads
File
Category
Size
Filetype
File IHC mouse antibody
Category Protocol
Size 167.19 KB
Filetype pdf
File Immunofluorescence Protocol
Category Protocol
Size 178.25 KB
Filetype pdf
Datasheet
MSDS
FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.
PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

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