anti-T Cells (CD43) mouse monoclonal, MT1, supernatant
- Mouse monoclonal
- Suitable for IHC on frozen and paraffin sections and WB
- Reacts with human
- Isotype: IgG1
|Purification||Hybridoma cell culture supernatant|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Isolated from human lymph node cells|
|Formulation||Contains 0.09% sodium azide|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:5-1:20|
|Immunohistochemistry (IHC) - paraffin||1:5-1:20 (microwave treatment recommended)|
|Western Blot (WB)||Assay dependent|
CD43 is a 95/100 – 135 kD cell-surface glycoprotein, expressed in high amounts on all leucocytes (except most resting B-lymphocytes). MT1 is an excellent marker for acute lymphoblastic lymphomas; the antibody is reactive with all T-cells, immature B-cells, histiocytes and myeloid cells. In Western blot analysis MT1 binds to glycoproteins of 95/100, 110 and 190 kD.
Positive control: Tonsil.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at email@example.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.