anti-TNF-alpha mouse monoclonal, EBS-O-175, purified

Cat. No. 691669

Available, delivery time 1-7 days

€340.00*

Key Features
  • Mouse monoclonal
  • Suitable for ELISA, FACS, ICC/IF, IHC and WB
  • Reacts with human
  • Isotype: IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application ELISA, FACS, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Human
No reactivity Mouse
Storage 2-8°C
Intended use Research use only
Clone EBS-O-175
Immunogen Human rTNF-alpha
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P01375 (Human)
Synonym Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a [Cleaved into: Tumor necrosis factor, membrane form, N-terminal fragment, NTF; Intracellular domain 1, ICD1; Intracellular domain 2, ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form], TNF, TNFA, TNFSF2
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
ELISA Assay dependent
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Western Blot (WB) 1:50-1:100 (1-2 µg/ml)
Background

EBS-O-175 reacts with human TNF-alpha and not with mouse TNF-alpha. This antibody neutralises Hu rTNF-alpha mediated cytotoxicity of L929 cells and inhibits tumor growth in mice. It protects mice against toxicity of Hu rTNF-alpha.

Positive control: HeLa, HL-60, or A431 cells. Macrophages in lymph node or tonsil.

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