Western blot analysis of recombinant human Uroplakin 3A (UPK3A) lysate and recombinant human Uroplakin 3B (UPK3B) lysate with anti-Uroplakin III antibody. Western blot analysis was performed on 0.05 µg UPK3A and 0.05 µg UPK3B. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-Uroplakin III mouse monoclonal, AU1 (Cat. No. 610108) was diluted in blocking buffer (antibody concentration 0.05 µg/ml) and incubated at RT for 1 h. The secondary antibody goat anti-mouse HRP was also diluted in blocking buffer (antibody concentration 0.2 µg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate. The antibody recognizes UPK3A but not UPK3B.
Western blot analysis of recombinant human Uroplakin 3A (UPK3A) lysate and recombinant human Uroplakin 3B (UPK3B) lysate with anti-Uroplakin III antibody. Western blot analysis was performed on 0.05 µg UPK3A and 0.05 µg UPK3B. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-Uroplakin III mouse monoclonal, AU1 (Cat. No. 610108) was diluted in blocking buffer (antibody concentration 0.05 µg/ml) and incubated at RT for 1 h. The secondary antibody goat anti-mouse HRP was also diluted in blocking buffer (antibody concentration 0.2 µg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate. The antibody recognizes UPK3A but not UPK3B.
- Purified, liquid, small
- Mouse monoclonal
- Suitable for IHC and WB
- Reacts with bovine, human, pig and rat
- Isotype: IgG1
- The antibody recognizes UPK3A but not UPK3B
Product description
| Quantity | 200 µl |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Application | IHC, WB |
| Purification | Affinity chromatography |
| Reactivity | Bovine, Human, Pig, Rat |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | AU1 |
| Immunogen | Asymmetric unit membrane (AUM) preparation from bovine urinary bladder |
| Concentration | 100 µg/ml |
| Formulation | 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4 |
| UniprotID | P38574 (Bovine), O75631 (Human), A0A4X1W3H8 (Pig), D3ZZ76 (Rat) |
| Synonym | Uroplakin-3a, UP3a, Uroplakin III, UPIII, UPK3A, UPK3 |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - paraffin | 1:20-1:60 (1.6-5 µg/ml; microwave treatment recommended) |
| Western Blot (WB) | 1:4,000-1:20,000 (0.005-0.025 µg/ml) |
Background
Mab AU1 reacts specifically with uroplakin IIIA present in the superficial cell layer of the urothelium. The binding region has been localized on the extracellular part of the antigen. Together with the uroplakins UP Ia, UP Ib and UP II, uroplakin III contributes in constituting the asymmetrical unit membrane of the plaques of urothelial superficial (umbrella) cells. Uroplakin is a membrane glycoprotein (47 kDa) and has been shown to be a specific marker of terminal urothelial differentia-tion (Wu et al. 1993 & 1194).
Antibody AU1 strongly stains the urothelial surface membrane in paraffin sections of human renal pelvis, ureter, bladder, and urethra.
About 60% of human transitional cell carcinomas (including metastases) maintain focal (sometimes very limited) expression of uroplakin III. Until now, no uroplakin staining was found in any non-urothelial carcinoma (Moll et al. 1995).
Uroplakin III may thus serve as a specific urothelial differentiation marker in cases of metastatic carcinomas with unclear primary tumor.
Moll, R., Wu, X.-R., Lin, J.-H. & Sunt, T.-T. Uroplakins, Specific Membrane Proteins of Urothelial Umbrella Cells, as Histological Markers of Metastatic Transitional Cell Carcinomas. Am. J. Pathol. 147, 1383-1397 (1995).
Wu, X.-R. et al. Mammalian Uroplakins: A group of highly conserved urothelial differentiation-related membrane proteins. J. Biol. Chem. 269, 13716-13724 (1994).
Wu, X. R. & Sun, T. T. Molecular cloning of a 47 kDa tissue-specific and differentiation-dependent urothelial cell surface glycoprotein. J. Cell Sci. 106, 31-43 (1993).
References/Publications (4)
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FAQs
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
- Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
- Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
- Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
- Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.