anti-Vasoactive Intestinal Peptide rabbit polyclonal, serum

Cat. No. 16099

Available, delivery time 1-7 days

€423.00*

Key Features
  • Rabbit polyclonal
  • Suitable for IHC on frozen and paraffin sections
  • Reacts with cat, human, pig and rat
Product description
Quantity 50 µl (lyoph.)
Antibody Type Polyclonal
Host Rabbit
Conjugate Unconjugated
Application IHC
Purification Undiluted antiserum
Reactivity Cat, Human, Pig, Rat
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Purified porcine VIP
Formulation Lyophilized; reconstitute in 100 µl dist. water
UniprotID A0A5F5XTM9 (Cat, Felis silvestris catus), P01282 (Human), P01284 (Pig), P01284 (Pig), P04566 (Guinea pig), P01283 (Rat)
Synonym VIP peptides [Cleaved into: Intestinal peptide PHV-42, Peptide histidine valine 42; Intestinal peptide PHM-27, Peptide histidine methioninamide 27; Vasoactive intestinal peptide, VIP, Vasoactive intestinal polypeptide], VIP
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:1,500-1:5,000
Immunohistochemistry (IHC) - paraffin 1:1,500-1:5,000 (microwave treatment recommended)
Background

VIP is localized in nerve fibers of the central and peripheral nervous system, and is probably acting as a neurotransmitter. Smooth muscle relaxation, vasodilation and secretion from exocrine glands are some of the effects of VIP. The Verner-Morrison or Watery Diarrhea Hypokaliemia and Achlorhydria (WDHA) syndrome is a characteristic clinical syndrome associated with overproduction of VIP from endocrine tumors. These VIP-producing tumors are usually neuroblastomas of endocrine tumors in the pancreas.
Absorption with 10-100 ug immunogen per ml diluted antiserum abolishes the staining, while PHI, secretin, glucagon, GIP, and CCK do not.

Positive control: formalin-fixed paraffin sections of cat ileum.

References/Publications (4)
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