anti-Vasoactive Intestinal Peptide rabbit polyclonal, serum

Cat. No. 16099

Available, delivery time 1-7 days


Key Features
  • Rabbit polyclonal
  • Suitable for IHC on frozen and paraffin sections
  • Reacts with cat, human, pig and rat
Product description
Quantity 50 µl (lyoph.)
Antibody Type Polyclonal
Host Rabbit
Conjugate Unconjugated
Application IHC
Purification Undiluted antiserum
Reactivity Cat, Human, Pig, Rat
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Purified porcine VIP
Formulation Lyophilized; reconstitute in 100 µl dist. water
UniprotID A0A5F5XTM9 (Cat, Felis silvestris catus), P01282 (Human), P01284 (Pig), P01284 (Pig), P04566 (Guinea pig), P01283 (Rat)
Synonym VIP peptides [Cleaved into: Intestinal peptide PHV-42, Peptide histidine valine 42; Intestinal peptide PHM-27, Peptide histidine methioninamide 27; Vasoactive intestinal peptide, VIP, Vasoactive intestinal polypeptide], VIP
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:1,500-1:5,000
Immunohistochemistry (IHC) - paraffin 1:1,500-1:5,000 (microwave treatment recommended)

VIP is localized in nerve fibers of the central and peripheral nervous system, and is probably acting as a neurotransmitter. Smooth muscle relaxation, vasodilation and secretion from exocrine glands are some of the effects of VIP. The Verner-Morrison or Watery Diarrhea Hypokaliemia and Achlorhydria (WDHA) syndrome is a characteristic clinical syndrome associated with overproduction of VIP from endocrine tumors. These VIP-producing tumors are usually neuroblastomas of endocrine tumors in the pancreas.
Absorption with 10-100 ug immunogen per ml diluted antiserum abolishes the staining, while PHI, secretin, glucagon, GIP, and CCK do not.

Positive control: formalin-fixed paraffin sections of cat ileum.

References/Publications (4)
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The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

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