anti-Vimentin mouse monoclonal, VIM 3B4, liquid, purified

Liquid

1 ml (50 µg/ml)

Liquid

200 µl (50 µg/ml)

Lyophilized

50 µg

FITC

250 µl

Prediluted

5 ml

Cat. No. 690013

Prices excl. VAT

Available, delivery time: 1-7 days

€207.00*

Datasheet

Key Features

Purified, liquid
Mouse monoclonal
Suitable for ICC/IF, IHC and WB
Reacts with amphibia, bovine, chicken, human, mouse and monkey
Isotype: IgG2a

Product description

Quantity	1 ml
Antibody Type	Monoclonal
Host	Mouse
Isotype	IgG2a kappa
Conjugate	Unconjugated
Application	ICC/IF, IHC, WB
Purification	Affinity chromatography
Reactivity	Amphibia, Bovine, Chicken, Human, Monkey, Mouse
Storage	Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use	Research use only
Clone	VIM 3B4
Immunogen	Vimentin purified from bovine lens
Concentration	50 µg/ml (50 µg)
Formulation	PBS pH 7.4 with 0.09% sodium azide and 0.5% BSA
UniprotID	P48616 (Bovine), P09654 (Chicken), P08670 (Human)
Synonym	Vimentin, VIM

Applications

Tested applications	Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	Assay dependent
Immunohistochemistry (IHC) - frozen	1:100-1:200 (250-500 ng/ml)
Immunohistochemistry (IHC) - paraffin	1:100-1:200 (250-500 ng/ml, protease treatment and/or microwave treatment recommended)
Western Blot (WB)	1:500-1:5,000 (10-100 ng/ml)

Background

The antibody is highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. VIM 3B4 has turned out to be the most avid mab to vimentin.
Polypeptide reacting: 57 kDa intermediate filament protein (vimentin) of mesenchymal cells.
Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma.
The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al.(1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995).

Tested cultured cell lines: fibroblasts (SV-80).

Bohn W, Wiegers W, Beuttenmüller M, Traub P: Species-specific recognition patterns of monoclonal antibodies directed against vimentin. Exp Cell Res 201: 1-7 (1992).
Rogers KR, Eckelt A, Nimmrich V, Janssen K-P, Schliwa M, Herrmann H, Franke WW: Truncation mutagenesis of the non-alpha-helical carboxyterminal tail domain of vimentin reveals contributions to cellular localization but not to filament assembly. Eur J Cell Biol 66: 136-150 (1995).

References/Publications (23)

Publication

Species

Application

Publication Soglia, F. et al. The evolution of vimentin and desmin in Pectoralis major muscles of broiler chickens supports their essential role in muscle regeneration. Front. Physiol. 13, (2022).

Species chicken

Application WB

Publication Soglia, F. et al. Distribution and Expression of Vimentin and Desmin in Broiler Pectoralis major Affected by the Growth-Related Muscular Abnormalities. Front.Physiol. 10, 1581 (2020)

Species chicken

Application WB,IHC (frozen),IHC

Publication Cossu, G. et al. An exceptional presentation of pituicytoma apoplexy: A case report. Oncol.Lett. 16, 643-647 (2018)

Species human

Application IHC (paraffin)

Publication Aguirre-Portolés, C., et al. ABCA1 overexpression worsens colorectal cancer prognosis by facilitating tumour growth and caveolin-1-dependent invasiveness, and ... Mol. Oncol. 12, 1735–1752 (2018).

Species human

Application ICC-IF

Publication Lever, M., Brand-Saberi, B. & Theiss, C. Neurogenesis, gliogenesis and the developing chicken optic tectum: an immunohistochemical and ultrastructural analysis. Brain Struct. Funct. 219, 1009-1024 (2014).

Species chicken

Application IHC (frozen)

Publication Zayas-Santiago, A. et al. Unidirectional photoreceptor-to-Müller glia coupling and unique K+ channel expression in Caiman retina. PLoS One 9, (2014).

Species caiman

Application IHC

Publication Heid, H. et al. On the formation of lipid droplets in human adipocytes: the organization of the perilipin-vimentin cortex. PLoS One 9, e90386 (2014).

Species human

Application WB,ICC-IF

Publication Rickelt, S. et al. A novel kind of tumor type-characteristic junction: plakophilin-2 as a major protein of adherens junctions in cardiac myxomata. Mod. Pathol. 23, 1429-1437 (2010).

Species human

Application WB

Publication Martinez-Iglesias, O., Garcia-Silva, S., Regadera, J. & Aranda, A. Hypothyroidism Enhances Tumor Invasiveness and Metastasis Development. PLoS One 4, (2009).

Species human

Application IHC (paraffin)

Publication Straub, B. K., Stoeffel, P., Heid, H., Zimbelmann, R. & Schirmacher, P. Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 47, 1936-1946 (2008).

Species human

Application IHC (frozen)

Publication Moll, R. et al. The cardiac isoform of ?-actin in regenerating and atrophic skeletal muscle, myopathies and rhabdomyomatous tumors: an immunohistochemical study using monoclonal antibodies. Virchows Arch. 449, 175-191 (2006).

Species human

Application IHC (paraffin)

Publication Bär, H. et al. Severe muscle disease-causing desmin mutations interfere with in vitro filament assembly at distinct stages. Proc. Natl. Acad. Sci. U. S. A. 102, 15099-104 (2005).

Species human

Application ICC-IF

Publication Koeser, J., Troyanovsky, S. M., Grund, C. & Franke, W. W. De novo formation of desmosomes in cultured cells upon transfection of genes encoding specific desmosomal components. Exp. Cell Res. 285, 114-30 (2003).

Species human

Application ICC-IF

Publication Akat, K. et al. Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol. 106, 337-347 (2003).

Species human

Application IHC (frozen)

Publication Riedel, I. et al. Brenner tumors but not transitional cell carcinomas of the ovary show urothelial differentiation: immunohistochemical staining of urothelial markers, including cytokeratins and uroplakins. Virchows Arch. 438, 181-91 (2001).

Species human

Application IHC (paraffin)

Publication Moll, I., Houdek, P., Schäfer, S., Nuber, U. & Moll, R. Diversity of desmosomal proteins in regenerating epidermis: immunohistochemical study using a human skin organ culture model. Arch. Dermatol. Res. 291, 437-46 (1999).

Species human

Application IHC (frozen)

Publication Gomi, H. et al. Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions. Neuron 14, 29-41 (1995).

Species human

Application IHC (paraffin)

Publication Demirkesen, C., Hoede, N. & Moll, R. Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J. Cutan. Pathol. 22, 518-35 (1995).

Species human

Application IHC (paraffin)

Publication Herrmann, H., Eckelt, A., Brettel, M., Grund, C. & Franke, W. W. Temperature-sensitive Intermediate Filament Assembly. J. Mol. Biol. 234, 99-113 (1993).

Species human,xenopus

Application ICC-IF

Publication Bohn, W., Wiegers, W., Beuttenmüller, M. & Traub, P. Species-specific recognition patterns of monoclonal antibodies directed against vimentin. Exp. Cell Res. 201, 1-7 (1992).

Species human,monkey,rabbit,bovine,chicken,dog,equine

Application WB,ICC-IF

Publication Knapp, A. C. & Franke, W. W. Spontaneous losses of control of cytokeratin gene expression in transformed, non-epithelial human cells occurring at different levels of regulation. Cell 59, 67-79 (1989).

Species human

Application WB,ICC-IF

Publication Rungger-Brandle, E., Achtstätter, T. & Franke, W. W. An epithelium-type cytoskeleton in a glial cell: Astrocytes of amphibian optic nerves contain cytokeratin filaments and are connected by desmosomes. J. Cell Biol. 109, 705-716 (1989).

Species xenopus

Application IHC (frozen)

Publication Herrmann, H., Fouquet, B. & Franke, W. W. Expression of intermediate filament proteins during development of Xenopus laevis. I. cDNA clones encoding different forms of vimentin. Development 105, 279-98 (1989).

Species xenopus

Application WB,IHC (frozen),ICC-IF

Downloads

File

Category

Size

Filetype

File IHC mouse antibody

Category Protocol

Size 167.19 KB

Filetype pdf

File WB protocol

Category Protocol

Size 210.69 KB

Filetype pdf

Datasheet

MSDS

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FAQs

The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.

PROGEN offers different formats for mouse monoclonal antibodies. Different antibodies may not be offered in each format, the following are all possible formats:

Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.

We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.

PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

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