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anti-AAV VP1 mouse monoclonal, A1, lyophilized, purified

A1 reacts with VP1 of AAV1-12. In immunoprecipitation, an occasional reaction with a non-AAV-derived protein is found.
Cat. No.: 61056
Quantity:  50 µg

Delivery Time: usually 1-7 working days

$450.00
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a
Clone A1
Immunogen AAV2 capsids
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml sterile PBS
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAV11, AAV12

Applications

Tested applications Tested dilutions
ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunoprecipitation (IP) assay dependent
Western Blot (WB) 1:500

Background

Details

A1 reacts with VP1 of adeno-associated virus 1-12 (AAV1-12). In immunoprecipitation, an occasional reaction with a non-AAV-derived protein is found. Epitope mapping experiments (Wobus et al. 2000) identified aa123 to aa131 of VP1 capsid protein as the specific binding region.

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Reference

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