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anti-AAV VP1/VP2 mouse monoclonal, A69, lyophilized, purified

Recognizes AAV2 viral capsid proteins VP1 and VP2, which are highly enriched in the nucleus.
Cat. No.: 61057
Quantity:  50 µg

Delivery Time: usually 1-7 working days

$427.00
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone A69
Immunogen AAV2 capsids
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity AAV2

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunoprecipitation (IP) assay dependent
Western Blot (WB) 1:500

Background

Details

A69 reacts with VP1 and V 2 of adeno-associated virus 2 (AAV2) which are highly enriched in the nucleus. Epitope mapping experiments (Wobus et al. 2000) identified aa169 to aa184 of VP2 and (with reduced intensity) aa123 to aa136 of VP1 capsid proteins as the specific binding region.

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Reference

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