Alternative Products
Cat. No. | Product Name | Format | Quantity | Price |
---|---|---|---|---|
65158 |
anti-AAV VP1/VP2/VP3 mouse monoclonal, B1 |
supernatant | 5 mL |
$265.00
Excl. VAT, excl. Shipping Cost
|
61058S |
anti-AAV VP1/VP2/VP3 mouse monoclonal, B1 |
lyophilized, purified, sample | 10 µg |
$128.00
Excl. VAT, excl. Shipping Cost
|
61058-488 |
Anti-AAV VP1/VP2/VP3 mouse monoclonal, B1 |
AFDyeTM 488 Conjugate | 50 µg (100 µl) |
$498.00
Excl. VAT, excl. Shipping Cost
|
61058-647 |
Anti-AAV VP1/VP2/VP3 mouse monoclonal, B1 |
AFDyeTM 647 Conjugate | 50 µg (100 µl) |
$498.00
Excl. VAT, excl. Shipping Cost
|
Product description
Host | mouse |
---|---|
Antibody Type | monoclonal |
Isotype | IgG1 |
Clone | B1 |
Immunogen | AAV2 capsids |
Purification | protein A affinity chromatography |
Conjugate | unconjugated |
Formulation | lyophilized; reconstitute in 1 ml sterile PBS |
Storage | lyophilized antibody: 2-8°C; reconstituted antibody: short term at 2-8°C; for long term storage we recommend to add 0.5% BSA prior to storing in aliquots at -20°C; avoid freeze/thaw cycles |
Intended use | Research use only |
Tested species reactivity | AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAVDJ |
Applications
Tested applications | Tested dilutions |
---|---|
Affinity Chromatography | assay dependent |
Dot Blot | 1:500 (0.1 µg/ml; denaturing conditions) |
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
Immunoprecipitation (IP) | assay dependent (precipitation of mainly free VP proteins) |
Western Blot (WB) | 1:250 - 1:500 (0.1 - 0.2 µg/ml) |
Background
Details
B1 reacts with free VP1, VP2 and VP3 of adeno-associated virus (AAV) and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus et al. 2000) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection.
Reference
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Species
Application
AAV1
AAV2
AAV3
AAV5
AAV6
AAV7
AAV8
AAV9
AAV2
AAV3
AAV5
AAV6
AAV7
AAV8
AAV9
dot blot
AAV1
AAV2
AAV3
AAV5
AAV6
AAV7
AAV8
AAV9
AAVrh10
AAV2
AAV3
AAV5
AAV6
AAV7
AAV8
AAV9
AAVrh10
WB
AAV2
WB
dot blot
dot blot
AAV1
AAV2
AAV5
AAV8
AAV9
AAV2
AAV5
AAV8
AAV9
WB
AAV1
AAV5
AAV6
AAV5
AAV6
WB
ICC-IF
ICC-IF
AAV2
WB
IP
ICC-IF
IP
ICC-IF