WB with anti-AAV VP1/VP2/VP3 antibody (Cat. No. 61058, 1:250) using AAV2 capsids as sample

anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, lyophilized, purified

Useful for immunolocalization studies of capsid formation in combination with Cat. No. 61055 and rabbit anti-AAV (Cat. No. 61084). Reacts with free VP1, VP2 and VP3 and at a very reduced level with assembled capsids. Recognizes all published AAV serotypes (AAV4, 11 & 12, no cross-reaction)

Cat. No.: 61058
Quantity: 50 µg

Delivery Time: usually 1-7 working days

$450.00

Excl. 19% Tax, excl. Shipping Cost

Qty:

Alternative Products

Cat. No.	Product Name	Format	Quantity	Price
65158	anti-AAV VP1/VP2/VP3 mouse monoclonal, B1	supernatant	5 mL	$259.00 Excl. 19% Tax, excl. Shipping Cost
61058S	anti-AAV VP1/VP2/VP3 mouse monoclonal, B1	lyophilized, purified, sample	10 µg	$128.00 Excl. 19% Tax, excl. Shipping Cost

Product description

Host	mouse
Antibody Type	monoclonal
Isotype	IgG1
Clone	B1
Immunogen	adeno-associated virus 2 capsid proteins and virus particles
Purification	protein A affinity chromatography
Conjugate	unconjugated
Formulation	lyophilized; reconstitute in 1 ml sterile PBS
Storage	short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use	Research use only
Tested species reactivity	AAV2 (published cross-reactivity with all published AAV serotypes (AAV4, 11 &12, no cross-reaction))

Applications

Tested applications	Tested dilutions
Affinity Chromatography	assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	assay dependent
Immunohistochemistry (IHC) - frozen	1:10
Immunohistochemistry (IHC) - paraffin	1:10 (microwave treatment recommended)
Immunoprecipitation (IP)	assay dependent (mainly free VP proteins)
Western Blot (WB)	1:250

Background

Details

Useful for immunolocalization studies of capsid formation in combination with monoclonal antibody A20 (Cat. No. 61055/ 65155) and rabbit poly-clonal antibody to AAV capsid proteins (Cat. No. 61084). B1 reacts with free VP 1, VP 2 and VP 3 of adeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus et al. 2000) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection.

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Reference

Publication	Species	Application
Wu, Y. et al. A Recombinant Baculovirus Efficiently Generates Recombinant Adeno-Associated Virus Vectors in Cultured Insect Cells and Larvae. Mol.Ther.Methods.Clin.Dev. 10, 38-47 (2018).	AAV2	WB
Schiller, L. et al. Enhanced Production of Exosome-Associated AAV by Overexpression of the Tetraspanin CD9. Mol.Ther.Methods.Clin.Dev. 9, 278-287 (2018).	AAV2	WB
Tseng, Y.-S. et al. Generation and characterization of anti-Adeno-associated virus serotype 8 (AAV8) and anti-AAV9 monoclonal antibodies. J. Virol. Methods 236, 105–110 (2016).	AAV, 1, 2, 3, 5, 6, 7, 8, 9	dot blot
Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1–12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212–222 (2014).	AAV1, 2, 3, 5, 6, 7, 8, 9, 10	WB
Jin, L.-F. et al. Ultrasound Targeted Microbubble Destruction Stimulates Cellular Endocytosis in Facilitation of Adeno-Associated Virus Delivery. Int. J. Mol. Sci 14, 9737–9750 (2013).	AAV2	WB
Naumer, M. et al. Properties of the Adeno-Associated Virus Assembly-Activating Protein. J. Virol. 86, 13038–13048 (2012).	AAV2	WB, dot blot
Sonntag, F. et al. The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes. J. Virol. 85, 12686–12697 (2011).	AAV1, 2, 5, 8, 9	WB
Fragkos, M., Breuleux, M., Clément, N. & Beard, P. Recombinant Adeno-Associated Viral Vectors Are Deficient in Provoking a DNA Damage Response. J. Virol. 82, 7379–7387 (2008).	AAV2	WB
Kuck, D., Kern, A. & Kleinschmidt, J. A. Development of AAV serotype-specific ELISAs using novel monoclonal antibodies. J. Virol. Methods 140, 17–24 (2007).	AAV1, 5, 6	WB, ICC-IF
Grimm, D., Kay, M. A. & Kleinschmidt, J. A. Helper virus-free, optically controllable, and two-plasmid-based production of adeno-associated virus vectors of serotypes 1 to 6. Mol. Ther. 7, 839–850 (2003).	AAV2	WB
Yan, Z. et al. Ubiquitination of both Adeno-Associated Virus Type 2 and 5 Capsid Proteins Affects the Transduction Efficiency of Recombinant Vectors. J. Virol. 76, 2043–2053 (2002).	AAV2, 5	WB
Zhang, H.-G. et al. Addition of Six-His-Tagged Peptide to the C Terminus of Adeno-Associated Virus VP3 Does Not Affect Viral Tropism or Production. J. Virol. 76, 12023–12031 (2002).	AAV2	WB
Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).		epitope mapping
Wistuba, A. et al. Subcellular Compartmentalization of Adeno-Associated Virus Type 2 Assembly. J. Virol. 71, 1341–1352 (1997).	AAV2	WB, IP, ICC-IF
Wistuba, A., Weger, S., Kern, A., Rgen, J. & Kleinschmidt, A. Intermediates of Adeno-Associated Virus Type 2 Assembly: Identification of Soluble Complexes Containing Rep and Cap Proteins. J. Virol. 69, 5311–5319 (1995).	AAV2	WB

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anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, lyophilized, purified

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