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anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, supernatant

Useful for immunolocalization studies of capsid formation in combination with Cat. No. 61055 and rabbit anti-AAV (Cat. No. 61084). Reacts with free VP1, VP2 and VP3 and at a very reduced degree with assembled capsids. Recognizes all published AAV serotypes (AAV4, 11 & 12, no cross-reaction)
Cat. No.: 65158
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€196.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone B1
Immunogen adeno-associated virus 2 capsid proteins and virus particles
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity AAV1, AAV10, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9 (negative with AAV4, 11, 12)

Applications

Affinity Chromatography assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use (after fixation with formaldehyde)
Immunoprecipitation (IP) assay dependent (mainly free VP proteins)
Western Blot (WB) assay dependent

Background

Details

Useful for immunolocalization studies of capsid formation in combination with monoclonal antibody A20 (Cat.No. 61055/65155) and rabbit polyclonal antibody to AAV capsid proteins (Cat.No. 61084). B1 reacts with free VP1, VP2 and VP3 of adeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus, C. E. et al., 2000) identified aa726 to aa733 (common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection.

Reference

Reference

  1. Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1–12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212–222 (2014).
  2. Naumer, M. et al. Properties of the Adeno-Associated Virus Assembly-Activating Protein. J. Virol. 86, 13038–13048 (2012).
  3. Sonntag, F. et al. The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes. J. Virol. 85, 12686–12697 (2011).
  4. Kuck, D. et al. Intranasal Vaccination with Recombinant Adeno-Associated Virus Type 5 against Human Papillomavirus Type 16 L1. J. Virol. 80, 2621–2630 (2006).
  5. Yan, Z. et al. Ubiquitination of both Adeno-Associated Virus Type 2 and 5 Capsid Proteins Affects the Transduction Efficiency of Recombinant Vectors. J. Virol. 76, 2043–2053 (2002).
  6. Zhang, H.-G. et al. Addition of Six-His-Tagged Peptide to the C Terminus of Adeno-Associated Virus VP3 Does Not Affect Viral Tropism or Production. J. Virol. 76, 12023–12031 (2002).
  7. Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).
  8. Sato, Y. et al. Detection of adeno-associated virus type 2 in patients with viral infection. Jpn. J. Infect. Dis. 52, 50–1 (1999).
  9. Wistuba, A. et al. Subcellular Compartmentalization of Adeno-Associated Virus Type 2 Assembly. J. Virol. 71, 1341–1352 (1997).
  10. Wistuba, A., Weger, S., Kern, A., Rgen, J. & Kleinschmidt, A. Intermediates of Adeno-Associated Virus Type 2 Assembly: Identification of Soluble Complexes Containing Rep and Cap Proteins. J. Virol. 69, 5311–5319 (1995).

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