Call +49 6221 8278-0

PROGEN - passion for research

close×

WB with anti-AAV VP1/VP2/VP3 antibody (Cat. No. 65158, 1:100) using AAV2 capsids as sample Zoom

WB with anti-AAV VP1/VP2/VP3 antibody (Cat. No. 65158, 1:100) using AAV2 capsids as sample

anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, supernatant

Useful for immunolocalization studies of capsid formation in combination with Cat. No. 61055 and rabbit anti-AAV (Cat. No. 61084). Reacts with free VP1, VP2 and VP3 and at a very reduced degree with assembled capsids. Recognizes all published AAV serotypes (AAV4, 11 & 12, no cross-reaction)
Cat. No.: 65158
Quantity:  5 mL

Delivery Time: usually 1-7 working days

$259.00
Excl. 19% Tax, excl. Shipping Cost

Call us now for more information on our products: +49 6221 8278-0

Any more questions?
PRODUCT REQUEST

Specifications

Alternative Products

Cat. No. Product Name Format Quantity Price
61058

anti-AAV VP1/VP2/VP3 mouse monoclonal, B1

lyophilized, purified 50 µg
$450.00
Excl. 19% Tax, excl. Shipping Cost
61058S

anti-AAV VP1/VP2/VP3 mouse monoclonal, B1

lyophilized, purified, sample 10 µg
$128.00
Excl. 19% Tax, excl. Shipping Cost

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone B1
Immunogen adeno-associated virus 2 capsid proteins and virus particles
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity AAV1, AAV10, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9 (negative with AAV4, 11, 12)

Applications

Tested applications Tested dilutions
Affinity Chromatography assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use (after fixation with formaldehyde)
Immunoprecipitation (IP) assay dependent (mainly free VP proteins)
Western Blot (WB) 1:25 - 1:200

Background

Details

Useful for immunolocalization studies of capsid formation in combination with monoclonal antibody A20 (Cat.No. 61055/65155) and rabbit polyclonal antibody to AAV capsid proteins (Cat.No. 61084). B1 reacts with free VP1, VP2 and VP3 of adeno-associated virus and at a very reduced degree with assembled capsids.
VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus, C. E. et al., 2000) identified aa726 to aa733 (common to all 3 VP proteins) as the specific binding region.
The antibody is also useful for characterization of different stages of infection.



Wobus CA, Hügle-Dörr B, Girod A, Petersen G, Hallek M, Kleinschmidt JA: Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: Epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J Virol 74, 9281-9293 (2000).

Reference

Material safety data sheet

Please complete the form below to request a material safety data sheet

Personal Info

* Required Fields

Product Request

Personal Info

* Required Fields