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WB with anti-AAV VP1/VP2/VP3 antibody (Cat. No. 65158, 1:100) using AAV2 capsids as sample Zoom

WB with anti-AAV VP1/VP2/VP3 antibody (Cat. No. 65158, 1:100) using AAV2 capsids as sample

anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, supernatant

Useful for immunolocalization studies of capsid formation in combination with Cat. No. 61055 and rabbit anti-AAV (Cat. No. 61084). Reacts with free VP1, VP2 and VP3 and at a very reduced degree with assembled capsids. Recognizes all published AAV serotypes (AAV4, 11 & 12, no cross-reaction)
Cat. No.: 65158
Quantity:  5 mL
No. of experiments:  ≤ 200 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$259.00
Excl. VAT, excl. Shipping Cost

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Specifications

Alternative Products

Cat. No. Product Name Format Quantity Price
61058

anti-AAV VP1/VP2/VP3 mouse monoclonal, B1

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61058S

anti-AAV VP1/VP2/VP3 mouse monoclonal, B1

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$128.00
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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone B1
Immunogen AAV2 capsids
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 200 (WB, 5 ml reaction volume)
Intended use Research use only
Tested species reactivity AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10

Applications

Tested applications Tested dilutions
Affinity Chromatography assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunoprecipitation (IP) assay dependent (mainly free VP proteins)
Western Blot (WB) 1:25 - 1:200

Background

Details

Useful for immunolocalization studies of capsid formation in combination with monoclonal antibody A20 (Cat.No. 61055/65155) and rabbit polyclonal antibody to AAV capsid proteins (Cat.No. 61084). B1 reacts with free VP1, VP2 and VP3 of adeno-associated virus (AAV) and at a very reduced degree with assembled capsids.
VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus, C. E. et al., 2000) identified aa726 to aa733 (common to all 3 VP proteins) as the specific binding region.
The antibody is also useful for characterization of different stages of infection.



Wobus CA, Hügle-Dörr B, Girod A, Petersen G, Hallek M, Kleinschmidt JA: Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: Epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J Virol 74, 9281-9293 (2000).

Reference

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