Alternative Products
Cat. No. | Product Name | Format | Quantity | Price |
---|---|---|---|---|
61058 |
anti-AAV VP1/VP2/VP3 mouse monoclonal, B1 |
lyophilized, purified | 50 µg |
$450.00
Excl. VAT, excl. Shipping Cost
|
61058S |
anti-AAV VP1/VP2/VP3 mouse monoclonal, B1 |
lyophilized, purified, sample | 10 µg |
$128.00
Excl. VAT, excl. Shipping Cost
|
61058-488 |
Anti-AAV VP1/VP2/VP3 mouse monoclonal, B1 |
AFDyeTM 488 Conjugate | 50 µg (100 µl) |
$498.00
Excl. VAT, excl. Shipping Cost
|
61058-647 |
Anti-AAV VP1/VP2/VP3 mouse monoclonal, B1 |
AFDyeTM 647 Conjugate | 50 µg (100 µl) |
$498.00
Excl. VAT, excl. Shipping Cost
|
Product description
Host | mouse |
---|---|
Antibody Type | monoclonal |
Isotype | IgG1 |
Clone | B1 |
Immunogen | AAV2 capsids |
Purification | hybridoma cell culture supernatant |
Conjugate | unconjugated |
Formulation | contains 0.09% sodium azide |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 200 (WB, 5 ml reaction volume) |
Intended use | Research use only |
Tested species reactivity | AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10 |
Applications
Tested applications | Tested dilutions |
---|---|
Affinity Chromatography | assay dependent |
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
Immunoprecipitation (IP) | assay dependent (mainly free VP proteins) |
Western Blot (WB) | 1:25 - 1:200 |
Background
Details
Useful for immunolocalization studies of capsid formation in combination with monoclonal antibody A20 (Cat.No. 61055/65155) and rabbit polyclonal antibody to AAV capsid proteins (Cat.No. 61084). B1 reacts with free VP1, VP2 and VP3 of adeno-associated virus (AAV) and at a very reduced degree with assembled capsids.
VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus, C. E. et al., 2000) identified aa726 to aa733 (common to all 3 VP proteins) as the specific binding region.
The antibody is also useful for characterization of different stages of infection.
Wobus CA, Hügle-Dörr B, Girod A, Petersen G, Hallek M, Kleinschmidt JA: Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: Epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J Virol 74, 9281-9293 (2000).
Reference
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AAV2
AAV3
AAV5
AAV6
AAV7
AAV9
AAV2
AAV3
AAV5
AAV6
AAV7
AAV8
AAV9
AAVrh10
dot blot
AAV2
AAV5
AAV8
AAV9
AAV5
AAV6
ICC-IF
IP
ICC-IF