WB with anti-AAV VP1/VP2/VP3 antibody (Cat. No. 65158, 1:100) using AAV2 capsids as sample

anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, supernatant

Useful for immunolocalization studies of capsid formation in combination with Cat. No. 61055 and rabbit anti-AAV (Cat. No. 61084). Reacts with free VP1, VP2 and VP3 and at a very reduced degree with assembled capsids. Recognizes all published AAV serotypes (AAV4, 11 & 12, no cross-reaction)

Cat. No.: 65158
Quantity: 5 mL
No. of experiments: ≤ 200 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$265.00

Excl. VAT, excl. Shipping Cost

Qty:

Publication

Species

Application

Zhang, R. et al. Divergent engagements between adeno-associated viruses with their cellular receptor AAVR. Nat.Commun. 10, 3760 (2019)

AAV

Blessing, D. et al. Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells. Mol.Ther.Methods.Clin.Dev. 13, 14-26 (2019)

human

Wu, Y. et al. Development of Versatile and Flexible Sf9 Packaging Cell Line-Dependent OneBac System for Large-Scale Recombinant Adeno-Associated Virus Production. Hum.Gene.Ther.Methods. 30, 172-183 (2019)

AAV2

Feiner, R. et al. rAAV Engineering for Capsid-Protein Enzyme Insertions and Mosaicism Reveals Resilience to Mutational, Structural and Thermal Perturbations. Int.J.Mol.Sci. 20, (2019)

AAV2

Le, D. et al. Adeno-associated virus capsid protein expression in Escherichia coli and chemically defined capsid assembly. Sci.Rep. 9, 18631 (2019)

AAV2

Savy, A. et al. Genetics instability of wtAAV2 genome and AAV promoter activities in the Baculovirus/Sf9 cells system. PLoS.One. 13, e0199866 (2018)

AAV2

Savy, A. et al. Impact of Inverted Terminal Repeat Integrity on rAAV8 Production Using the Baculovirus/Sf9 Cells System. Hum.Gene.Ther.Methods. 28, 277-289 (2017).

AAV8

Tseng, Y.-S. et al. Generation and characterization of anti-Adeno-associated virus serotype 8 (AAV8) and anti-AAV9 monoclonal antibodies. J. Virol. Methods 236, 105â110 (2016).

AAV1
AAV2
AAV3
AAV5
AAV6
AAV7
AAV9

dot blot

Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1â12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212â222 (2014).

AAV1
AAV2
AAV3
AAV5
AAV6
AAV7
AAV8
AAV9
AAVrh10

Naumer, M. et al. Properties of the Adeno-Associated Virus Assembly-Activating Protein. J. Virol. 86, 13038â13048 (2012).

AAV2

WB
dot blot

Sonntag, F. et al. The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes. J. Virol. 85, 12686â12697 (2011).

AAV1
AAV2
AAV5
AAV8
AAV9

Kuck, D., Kern, A. & Kleinschmidt, J. A. Development of AAV serotype-specific ELISAs using novel monoclonal antibodies. J. Virol. Methods 140, 17â24 (2007).

AAV1
AAV5
AAV6

WB
ICC-IF

Grimm, D., Kay, M. A. & Kleinschmidt, J. A. Helper virus-free, optically controllable, and two-plasmid-based production of adeno-associated virus vectors of serotypes 1 to 6. Mol. Ther. 7, 839â850 (2003).

AAV2

Yan, Z. et al. Ubiquitination of both Adeno-Associated Virus Type 2 and 5 Capsid Proteins Affects the Transduction Efficiency of Recombinant Vectors. J. Virol. 76, 2043â2053 (2002).

AAV2
AAV5

Zhang, H.-G. et al. Addition of Six-His-Tagged Peptide to the C Terminus of Adeno-Associated Virus VP3 Does Not Affect Viral Tropism or Production. J. Virol. 76, 12023â12031 (2002).

AAV2

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281â93 (2000)

AAV2

epitope mapping

Wistuba, A. et al. Subcellular Compartmentalization of Adeno-Associated Virus Type 2 Assembly. J. Virol. 71, 1341â1352 (1997).

AAV2

WB
IP
ICC-IF

Wistuba, A., Weger, S., Kern, A., Rgen, J. & Kleinschmidt, A. Intermediates of Adeno-Associated Virus Type 2 Assembly: Identification of Soluble Complexes Containing Rep and Cap Proteins. J. Virol. 69, 5311â5319 (1995).

AAV2

Alternative Products

Cat. No.	Product Name	Format	Quantity	Price
61058	anti-AAV VP1/VP2/VP3 mouse monoclonal, B1	lyophilized, purified	50 µg	$450.00 Excl. VAT, excl. Shipping Cost
61058S	anti-AAV VP1/VP2/VP3 mouse monoclonal, B1	lyophilized, purified, sample	10 µg	$128.00 Excl. VAT, excl. Shipping Cost
61058-488	Anti-AAV VP1/VP2/VP3 mouse monoclonal, B1	AFDyeTM 488 Conjugate	50 µg (100 µl)	$498.00 Excl. VAT, excl. Shipping Cost
61058-647	Anti-AAV VP1/VP2/VP3 mouse monoclonal, B1	AFDyeTM 647 Conjugate	50 µg (100 µl)	$498.00 Excl. VAT, excl. Shipping Cost

Product description

Host	mouse
Antibody Type	monoclonal
Isotype	IgG1
Clone	B1
Immunogen	AAV2 capsids
Purification	hybridoma cell culture supernatant
Conjugate	unconjugated
Formulation	contains 0.09% sodium azide
Storage	short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments	≤ 200 (WB, 5 ml reaction volume)
Intended use	Research use only
Tested species reactivity	AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10

Applications

Tested applications	Tested dilutions
Affinity Chromatography	assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	assay dependent
Immunoprecipitation (IP)	assay dependent (mainly free VP proteins)
Western Blot (WB)	1:25 - 1:200

Background

Details

Useful for immunolocalization studies of capsid formation in combination with monoclonal antibody A20 (Cat.No. 61055/65155) and rabbit polyclonal antibody to AAV capsid proteins (Cat.No. 61084). B1 reacts with free VP1, VP2 and VP3 of adeno-associated virus (AAV) and at a very reduced degree with assembled capsids.
VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus, C. E. et al., 2000) identified aa726 to aa733 (common to all 3 VP proteins) as the specific binding region.
The antibody is also useful for characterization of different stages of infection.

Wobus CA, Hügle-Dörr B, Girod A, Petersen G, Hallek M, Kleinschmidt JA: Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: Epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J Virol 74, 9281-9293 (2000).

Reference

Publication

Species

Application

Zhang, R. et al. Divergent engagements between adeno-associated viruses with their cellular receptor AAVR. Nat.Commun. 10, 3760 (2019)

AAV

Blessing, D. et al. Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells. Mol.Ther.Methods.Clin.Dev. 13, 14-26 (2019)

human

AAV2

Feiner, R. et al. rAAV Engineering for Capsid-Protein Enzyme Insertions and Mosaicism Reveals Resilience to Mutational, Structural and Thermal Perturbations. Int.J.Mol.Sci. 20, (2019)

AAV2

Le, D. et al. Adeno-associated virus capsid protein expression in Escherichia coli and chemically defined capsid assembly. Sci.Rep. 9, 18631 (2019)

AAV2

Savy, A. et al. Genetics instability of wtAAV2 genome and AAV promoter activities in the Baculovirus/Sf9 cells system. PLoS.One. 13, e0199866 (2018)

AAV2

Savy, A. et al. Impact of Inverted Terminal Repeat Integrity on rAAV8 Production Using the Baculovirus/Sf9 Cells System. Hum.Gene.Ther.Methods. 28, 277-289 (2017).

AAV8

Tseng, Y.-S. et al. Generation and characterization of anti-Adeno-associated virus serotype 8 (AAV8) and anti-AAV9 monoclonal antibodies. J. Virol. Methods 236, 105â110 (2016).

AAV1
AAV2
AAV3
AAV5
AAV6
AAV7
AAV9

dot blot

Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1â12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212â222 (2014).

AAV1
AAV2
AAV3
AAV5
AAV6
AAV7
AAV8
AAV9
AAVrh10

Naumer, M. et al. Properties of the Adeno-Associated Virus Assembly-Activating Protein. J. Virol. 86, 13038â13048 (2012).

AAV2

WB
dot blot

Sonntag, F. et al. The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes. J. Virol. 85, 12686â12697 (2011).

AAV1
AAV2
AAV5
AAV8
AAV9

Kuck, D., Kern, A. & Kleinschmidt, J. A. Development of AAV serotype-specific ELISAs using novel monoclonal antibodies. J. Virol. Methods 140, 17â24 (2007).

AAV1
AAV5
AAV6

WB
ICC-IF

AAV2

Yan, Z. et al. Ubiquitination of both Adeno-Associated Virus Type 2 and 5 Capsid Proteins Affects the Transduction Efficiency of Recombinant Vectors. J. Virol. 76, 2043â2053 (2002).

AAV2
AAV5

Zhang, H.-G. et al. Addition of Six-His-Tagged Peptide to the C Terminus of Adeno-Associated Virus VP3 Does Not Affect Viral Tropism or Production. J. Virol. 76, 12023â12031 (2002).

AAV2

epitope mapping

Wistuba, A. et al. Subcellular Compartmentalization of Adeno-Associated Virus Type 2 Assembly. J. Virol. 71, 1341â1352 (1997).

AAV2

WB
IP
ICC-IF

AAV2

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anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, supernatant

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