Dot blot with different AAV serotypes and rabbit polyclonal anti-AAV2 VP1/VP2/VP3 antibody (Courtesy of Regina Heilbronn, Charité Universitätsmedizin Berlin, Mietzsch et al. Hum Gene Ther. 2014 Mar 1; 25(3):212-222)

anti-AAV VP1/VP2/VP3 rabbit polyclonal (VP51), serum

Reacts with VP1, VP2 and VP3. Raised against recombinant AAV2 capsid proteins.

Cat. No.: 61084
Quantity: 250 µL
No. of experiments: ≤ 25 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$450.00

Excl. VAT, excl. Shipping Cost

Qty:

Publication

Species

Application

Grosse, S. et al. Relevance of Assembly-Activating Protein for Adeno-associated Virus Vector Production and Capsid Protein Stability in Mammalian and Insect Cells. J.Virol. 91,Â (2017).

AAV2

Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1â12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212â222 (2014).

AAV1
AAV2
AAV3
AAV4
AAV5
AAV6
AAV7
AAV8
AAV9
AAVrh10
AAV11
AAV12

dot blot

Naumer, M. et al. Properties of the Adeno-Associated Virus Assembly-Activating Protein. J. Virol. 86, 13038â13048 (2012).

AAV2

Sonntag, F. et al. The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes. J. Virol. 85, 12686â12697 (2011).

AAV1
AAV2
AAV3
AAV4
AAV5
AAV6
AAV8
AAV9

dot blot
ICC-IF

Kuck, D., Kern, A. & Kleinschmidt, J. A. Development of AAV serotype-specific ELISAs using novel monoclonal antibodies. J. Virol. Methods 140, 17â24 (2007).

AAV1
AAV2
AAV3
AAV4
AAV5
AAV6
AAV8
AAV9

WB
dot blot

Kuck, D. et al. Intranasal Vaccination with Recombinant Adeno-Associated Virus Type 5 against Human Papillomavirus Type 16 L1. J. Virol. 80, 2621â2630 (2006).

AAV5

Grimm, D., Kay, M. A. & Kleinschmidt, J. A. Helper virus-free, optically controllable, and two-plasmid-based production of adeno-associated virus vectors of serotypes 1 to 6. Mol. Ther. 7, 839â850 (2003).

AAV2

Product description

Host	rabbit
Antibody Type	polyclonal
Immunogen	AAV2 capsids
Purification	stabilized antiserum
Conjugate	unconjugated
Formulation	contains 0.09% sodium azide
Storage	short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments	≤ 25 (WB, 5 ml reaction volume)
Note	centrifuge prior to opening
Intended use	Research use only
Tested species reactivity	AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAV11, AAV12

Applications

Tested applications	Tested dilutions
ELISA	assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	1:50 – 1:100
Immunoprecipitation (IP)	1:10
Western Blot (WB)	1:200 – 1:500

Background

Details

The antibody reacts with VP1, VP2, and VP3 of adeno-associated virus (AAV).
Rabbit polyclonal antibody (VP51).

Reference

Publication

Species

Application

Grosse, S. et al. Relevance of Assembly-Activating Protein for Adeno-associated Virus Vector Production and Capsid Protein Stability in Mammalian and Insect Cells. J.Virol. 91,Â (2017).

AAV2

Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1â12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212â222 (2014).

AAV1
AAV2
AAV3
AAV4
AAV5
AAV6
AAV7
AAV8
AAV9
AAVrh10
AAV11
AAV12

dot blot

Naumer, M. et al. Properties of the Adeno-Associated Virus Assembly-Activating Protein. J. Virol. 86, 13038â13048 (2012).

AAV2

Sonntag, F. et al. The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes. J. Virol. 85, 12686â12697 (2011).

AAV1
AAV2
AAV3
AAV4
AAV5
AAV6
AAV8
AAV9

dot blot
ICC-IF

Kuck, D., Kern, A. & Kleinschmidt, J. A. Development of AAV serotype-specific ELISAs using novel monoclonal antibodies. J. Virol. Methods 140, 17â24 (2007).

AAV1
AAV2
AAV3
AAV4
AAV5
AAV6
AAV8
AAV9

WB
dot blot

Kuck, D. et al. Intranasal Vaccination with Recombinant Adeno-Associated Virus Type 5 against Human Papillomavirus Type 16 L1. J. Virol. 80, 2621â2630 (2006).

AAV5

AAV2

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anti-AAV VP1/VP2/VP3 rabbit polyclonal (VP51), serum

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anti-AAV VP1/VP2/VP3 rabbit polyclonal (VP51), serum

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