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anti-AAV1 (intact particle) mouse monoclonal, ADK1a, Biotin Conjugate

Specifically detects AAV1 intact virus particles, both empty and full capsids. ADK1a recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins. Not suitable for Immunoblotting. In immunofluorescence, a week cross-reactivity was observed with AAV6. No reaction with AAV type 2, 3, 4, 5
Cat. No.: 615150
Quantity:  750 µL

Delivery Time: usually 1-7 working days

€212.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a lambda
Clone ADK1a
Immunogen adeno-associated virus type 1 (AAV1) capsid proteins and virus particles
Purification affinity chromatography
Conjugate biotin
Formulation lyophilized; reconstitute with 750 μL dist. water (contains 0.09% sodium azide, antibody concentration after reconstitution 2.5 µg/ml)
Storage store at 2 – 8 °C
Tested species reactivity AAV1 (weak cross-reaction with AAV6)
Intended use Research use only

Applications

Tested applications Tested dilutions
ELISA 1:20
Immunohistochemistry (IHC) - frozen assay dependent
Immunohistochemistry (IHC) - paraffin assay dependent (microwave treatment recommended)

Background

Details

For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. ADK1a specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins.
The antibody cannot be used for immunoblotting.

Reference

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