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anti-AAV1 (intact particle) mouse monoclonal, ADK1a, lyophilized, purified

Specifically detects AAV1 intact virus particles, both empty and full capsids. ADK1a recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins. Not suitable for Immunoblotting. In immunofluorescence and dot plot analysis, a week cross-reactivity was observed with AAV6 and AAV12. No reaction with other AAV types.
Cat. No.: 610150
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€323.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a lambda
Clone ADK1a
Immunogen adeno-associated virus type 1 (AAV1) capsid proteins and virus particles
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity AAV1 (weak cross-reaction with AAV6 and 12)

Applications

Tested applications Tested dilutions
Affinity Chromatography assay dependent
Dot Blot assay dependent (non-denaturing conditions)
ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:20
Immunohistochemistry (IHC) - frozen assay dependent
Immunohistochemistry (IHC) - paraffin assay dependent (microwave treatment recommended)
Immunoprecipitation (IP) 1:5

Background

Details

For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. ADK1a specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins.
The antibody cannot be used for immunoblotting.

Reference

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