PROGEN - passion for research

close×

anti-AAV2 (intact particle) mouse monoclonal, A20, lyophilized, purified

Specifically detects intact virus particles, both empty and full capsids. With AAV2 and AAV3 A20 recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins. Not for WB.
Cat. No.: 61055
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€340.00
Excl. 19% Tax, excl. Shipping Cost

Call us now for more information on our products: +49 6221 8278-0

Any more questions?
PRODUCT REQUEST

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG3
Clone A20
Immunogen AAV2 capsid proteins and virus particles
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity AAV2 (in ELISA also applicable to AAV3)

Applications

Tested applications Tested dilutions
Affinity Chromatography assay dependent
ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:20
Immunohistochemistry (IHC) - frozen assay dependent
Immunohistochemistry (IHC) - paraffin assay dependent (microwave treatment recommended)
Immunoprecipitation (IP) 1:5
Neutralization Assay assay dependent

Background

Details

For characterization of different stages of infection and very useful for the analysis of the AAV2 assembly process. A20 specifically reacts with intact adeno-associated virus 2 particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unas-sembled capsid proteins. The antibody cannot be used for immunoblotting.

Epitope mapping experiments (Wobus et al. 2000) identified four immunoreactive (discontinous) regions. The major reaction was attributed to sequence aa 369 to aa378 of AAV2 capsids. The antibody is also useful for neutralizing experiments (Moskalenko et al. 2000).

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).
Moskalenko, M. et al. Epitope Mapping of Human Anti-Adeno-Associated Virus Type 2 Neutralizing Antibodies: Implications for Gene Therapy and Virus Structure. Jounal Virol. 74, 1761–1766 (2000).

Reference

Reference

PublicationSpeciesApplication
Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1–12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212–222 (2014). AAV2, 3 dot blot
Jin, L.-F. et al. Ultrasound Targeted Microbubble Destruction Stimulates Cellular Endocytosis in Facilitation of Adeno-Associated Virus Delivery. Int. J. Mol. Sci 14, 9737–9750 (2013). AAV2 ICC-IF
Naumer, M. et al. Properties of the Adeno-Associated Virus Assembly-Activating Protein. J. Virol. 86, 13038–13048 (2012). AAV2 ELISA, dot blot
Sonntag, F. et al. The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes. J. Virol. 85, 12686–12697 (2011). AAV2 ELISA, ICC-IF
Moskalenko, M. et al. Epitope Mapping of Human Anti-Adeno-Associated Virus Type 2 Neutralizing Antibodies: Implications for Gene Therapy and Virus Structure. Jounal Virol. 74, 1761–1766 (2000). AAV2 neutralization assay
Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000). AAV2, 3 ELISA, neutralization assay
Grimm, D. et al. Titration of AAV-2 particles via a novel capsid ELISA: packaging of genomes can limit production of recombinant AAV-2. Gene Ther. 6, 1322–30 (1999). AAV2 ELISA
Bartlett, J. S., Kleinschmidt, J., Boucher, R. C. & Samulski, R. J. Targeted adeno-associated virus vector transduction of nonpermissive cells mediated by a bispecific F(ab’gamma)2 antibody. Nat. Biotechnol. 17, 181–6 (1999). AAV2
Wistuba, A. et al. Subcellular Compartmentalization of Adeno-Associated Virus Type 2 Assembly. J. Virol. 71, 1341–1352 (1997). AAV2 ICC-IF, IP
Wistuba, A., Weger, S., Kern, A., Rgen, J. & Kleinschmidt, A. Intermediates of Adeno-Associated Virus Type 2 Assembly: Identification of Soluble Complexes Containing Rep and Cap Proteins. J. Virol. 69, 5311–5319 (1995). AAV2 IP

Material safety data sheet

Please complete the form below to request a material safety data sheet

Personal Info

* Required Fields

Product Request

Personal Info

* Required Fields