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Dot blot with different AAV serotypes, comparing the mouse monoclonal anti-AAV2 antibodies A20 and A20R (Master thesis Nathalie Müller, 2018) Zoom

Dot blot with different AAV serotypes, comparing the mouse monoclonal anti-AAV2 antibodies A20 and A20R (Master thesis Nathalie Müller, 2018)

anti-AAV2 (intact particle) mouse recombinant, A20R, lyophilized, purified

Specifically detects intact virus particles, both empty and full capsids. A20R recognizes a conformational epitope of assembled AA2 and AAV3 capsids. This epitope not present in denatured and native unassembled capsid proteins. Not for WB.
Cat. No.: 610298
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€340.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

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610298S

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Product description

Host mouse
Antibody Type recombinant
Isotype IgG1
Clone A20R
Immunogen AAV2 capsid proteins and virus particles
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml sterile PBS
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity AAV2 (in ELISA also applicable to AAV3)

Applications

Tested applications Tested dilutions
Dot Blot 1:500 (0.1 µg/ml)
ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:20
Immunoprecipitation (IP) 1:5
Neutralization Assay assay dependent

Background

Details

For characterization of different stages of infection and very useful for the analysis of the AAV2 assembly process. A20R specifically reacts with intact AAV2 particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unas-sembled capsid proteins. The antibody cannot be used for immunoblotting.
The antibody is also useful for neutralizing experiments.

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