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anti-AAV2 Replicase mouse monoclonal, 226.7, supernatant

Recognizes AAV replicase proteins Rep 78, Rep 68, Rep 52 and Rep 40 in AAV-infected cells. In Immunoblotting prominant reaction with Rep 78 and Rep 68, only faint reaction with Rep 52 and Rep 40.
Cat. No.: 65172
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€196.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone 226.7
Immunogen recombinant AAV2 Rep 78 protein, N-terminally truncated by 171 aa
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity AAV2

Applications

Tested applications Tested dilutions
Affinity Chromatography assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunoprecipitation (IP) assay dependent
Western Blot (WB) 1:2 - 1:50

Background

Details

Mab 226.7 reacts with Rep proteins (Rep78, Rep68, Rep52 and Rep40) of human AAV2-infected cells. The antibody shows low affinity which is most useful in affinity chromatography experiments where mild conditions can be used for dissociation of the antigen.
In WB it reacts mainly with Rep52 and Rep40, faint reaction with Rep78 and Rep68 (long incubation time and short washing steps are recommended).
Alternatives for WB: Cat. Nos 61069 /65169 and 61071 /65171 and for IF: Cat. Nos. 61073/65173

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