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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | 226.7 |
| Immunogen | recombinant AAV2 Rep 78 protein, N-terminally truncated by 171 aa |
| Purification | hybridoma cell culture supernatant |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 50 (WB, 5 ml reaction volume) |
| Intended use | Research use only |
| Tested species reactivity | AAV2 |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Affinity Chromatography | assay dependent |
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunoprecipitation (IP) | assay dependent |
| Western Blot (WB) | 1:2 - 1:50 |
Background
Details
Mab 226.7 reacts with Rep proteins (Rep78, Rep68, Rep52 and Rep40) of human AAV2-infected cells. The antibody shows low affinity which is most useful in affinity chromatography experiments where mild conditions can be used for dissociation of the antigen.
In WB it reacts mainly with Rep52 and Rep40, faint reaction with Rep78 and Rep68 (long incubation time and short washing steps are recommended).
Alternatives for WB: Cat. Nos 61069 /65169 and 61071 /65171 and for IF: Cat. Nos. 61073/65173
Reference
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