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anti-AAV2 Replicase mouse monoclonal, 303.9, lyophilized, purified

Recognizes AAV replicase proteins Rep 78, Rep 68, Rep 52 and Rep 40 in human AAV-2-infected cells. Cross-reactivity with AAV serotypes 1, 3, 5, 6, 7, 8,9 and 10 (4, 11 and 12 were negative). A weak cross-reactivity is seen in some human cell lines.
Cat. No.: 61069
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€346.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone 303.9
Immunogen recombinant AAV2 Rep 78 protein, N-terminally truncated by 171 aa
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity AAV2 (found in human and monkey)

Applications

Tested applications Tested dilutions
Western Blot (WB) 1:50 – 1:100

Background

Details

Mab 303.9 reacts with Rep proteins (Rep78, Rep68, Rep52 and Rep40) of human AAV2-infected cells, and also with serotypes 1,3,5, 6, 7, 8,9 and 10 (4, 11 and 12 negative) (Mietzsch et al. 2014). A weak cross-reactivity is seen in some human cell lines.

Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1–12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212–222 (2014).

Reference

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