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anti-AAV2 Replicase mouse monoclonal, 303.9, supernatant

Recognizes AAV replicase proteins Rep 78, Rep 68, Rep 52 and Rep 40 in human AAV-2-infected cells.Cross-reactivity with AAV serotypes 1, 3, 4, 5, 6, 7, 8 ,9 and 10 (4,11 and 12 were negative). A weak cross-reactivity is seen in some human cell lines.
Cat. No.: 65169
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€196.00
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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone 303.9
Immunogen recombinant AAV2 Rep 78 protein, N-terminally truncated by 171 aa
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity AAV1, AAV10, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9 (negative with AAV4, 11, 12)

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen assay dependent
Immunohistochemistry (IHC) - paraffin assay dependent
Western Blot (WB) 1:10

Background

Details

Mab 303.9 reacts with Rep proteins (Rep78, Rep68, Rep52 and Rep40) of human AAV2-infected cells, and also with serotypes 1,3,5, 6, 7, 8,9 and 10 (4, 11 and 12 negative) (Mietzsch, M. et al., 2014).
A weak cross-reactivity is seen in some human cell lines.



Mietzsch M, Grasse S, Zurawski C, Weger S, Bennett A, Agbandje-McKenna M, Muzyczka N, Zolotukhin S, Heilbronn R: OneBac: Platform for scalable and high-titer production of adeno-associated virus serotype 1-12 vectors for gene therapy. Hum Gene Ther 25, 212-222 (2014); doi: 10.1089/hum.2013.184

Reference

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