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anti-AAV2 Replicase mouse monoclonal, 303.9, supernatant

Recognizes AAV replicase proteins Rep 78, Rep 68, Rep 52 and Rep 40 in human AAV-2-infected cells.Cross-reactivity with AAV serotypes 1, 3, 4, 5, 6, 7, 8 ,9 and 10 (4,11 and 12 were negative). A weak cross-reactivity is seen in some human cell lines.
Cat. No.: 65169
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€196.00
Excl. 19% Tax, excl. Shipping Cost

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Cat. No. Product Name Format Quantity Price
61069

anti-AAV2 Replicase mouse monoclonal, 303.9

lyophilized, purified 50 µg
€346.00
Excl. 19% Tax, excl. Shipping Cost

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone 303.9
Immunogen recombinant AAV2 Rep 78 protein, N-terminally truncated by 171 aa
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity AAV1, AAV10, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9 (negative with AAV4, 11, 12)
Intended use Research use only

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen assay dependent
Immunohistochemistry (IHC) - paraffin assay dependent
Western Blot (WB) 1:10

Background

Details

Mab 303.9 reacts with Rep proteins (Rep78, Rep68, Rep52 and Rep40) of human AAV2-infected cells, and also with serotypes 1,3,5, 6, 7, 8,9 and 10 (4, 11 and 12 negative) (Mietzsch, M. et al., 2014).
A weak cross-reactivity is seen in some human cell lines.



Mietzsch M, Grasse S, Zurawski C, Weger S, Bennett A, Agbandje-McKenna M, Muzyczka N, Zolotukhin S, Heilbronn R: OneBac: Platform for scalable and high-titer production of adeno-associated virus serotype 1-12 vectors for gene therapy. Hum Gene Ther 25, 212-222 (2014); doi: 10.1089/hum.2013.184

Reference

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