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anti-AAV2 VP1 mouse monoclonal, A1, lyophilized, purified

A1 reacts with VP1 of AAV-2; weak cross-reaction with serotypes 1, 3, 4, 5, 6. In immunoprecipitation, an occasional reaction with a non-AAV-derived protein is found.
Cat. No.: 61056
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€340.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a
Clone A1
Immunogen adeno-associated virus 2 capsid proteins and virus particles
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity AAV2 (weak cross-reaction with serotypes 1, 3, 4, 5 and 6)

Applications

Tested applications Tested dilutions
ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:5 – 1:10
Immunohistochemistry (IHC) - paraffin 1:5 – 1:10 (microwave treatment recommended)
Immunoprecipitation (IP) assay dependent
Western Blot (WB) assay dependent

Background

Details

A1 reacts with VP1 of adeno-associated virus. In immunoprecipitation, an occasional reaction with a non-AAV-derived protein is found. Epitope mapping experiments (Wobus et al. 2000) identified aa123 to aa131 of VP1 capsid protein as the specific binding region.

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Reference

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