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anti-AAV2 VP1 mouse monoclonal, A1, supernatant

A1 reacts with VP1 of AAV-2; weak cross-reaction with serotypes 1, 3, 4, 5, 6. In immunoprecipitation, an occasional reaction with a non AAV-derived protein is found.
Cat. No.: 65156
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€196.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a
Clone A1
Immunogen adeno-associated virus 2 capsid proteins and virus particles
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.01% Thimerosal
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity AAV2 (weak cross-reaction with serotypes 1, 3, 4, 5 and 6)

Applications

ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Immunoprecipitation (IP) assay dependent
Western Blot (WB) assay dependent

Background

Details

A1 reacts with VP1 of AAV2. In immunoprecipitation, an occasional reaction with a non-AAV-derived protein is found. Epitope mapping experiments (Wobus et al. 2000) identified aa123 to aa131 of VP1 capsid protein as the specific binding region.

Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).

Reference

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