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Dot blot with different AAV serotypes and mouse monoclonal anti-AAV4 antibody, clone ADK4 (Courtesy of Regina Heilbronn, Charité Universitätsmedizin Berlin, Mietzsch et al. Hum Gene Ther. 2014 Mar 1; 25(3):212-222) Zoom

Dot blot with different AAV serotypes and mouse monoclonal anti-AAV4 antibody, clone ADK4 (Courtesy of Regina Heilbronn, Charité Universitätsmedizin Berlin, Mietzsch et al. Hum Gene Ther. 2014 Mar 1; 25(3):212-222)

anti-AAV4 (intact particle) mouse monoclonal, ADK4, supernatant

Specifically detects AAV4 intact virus particles, both empty and full capsids. ADK4 recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins. No cross-reaction with other AAV serotypes.
Cat. No.: 651147
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€196.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a kappa
Clone ADK4
Immunogen adeno-associated virus type 4 (AAV4) capsid proteins and virus particles
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity AAV4
Intended use Research use only

Applications

Tested applications Tested dilutions
ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Immunoprecipitation (IP) assay dependent

Background

Details

For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. ADK4 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins.
The antibody cannot be used for immunoblotting.

Reference

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