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anti-AAV5 (intact particle) Biotin Conjugate mouse monoclonal, ADK5a, lyophilized, purified

Specifically detects intact virus particles, both empty and full capsids. ADK5a recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins. No cross-reaction with other AAV serotypes. The antibody is not suitable for Immunoblotting.
Cat. No.: 615148
Quantity:  750 µL (concentration after reconstitution10 µg/ml)

Delivery Time: usually 1-7 working days

€212.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a kappa
Clone ADK5a
Immunogen adeno-associated virus type 5 (AAV5) capsid proteins and virus particles
Purification affinity chromatography
Conjugate biotin
Formulation lyophilized; reconstitute with 750 µl dist. water (final solution contains 0.09% sodium azide)
Storage store at 2 – 8 °C
Tested species reactivity AAV5 (found in human and monkey)

Applications

Tested applications Tested dilutions
ELISA 1:20 after reconstitution with 375 µl dist. water
Immunohistochemistry (IHC) - frozen assay dependent
Immunohistochemistry (IHC) - paraffin assay dependent (microwave treatment recommended)

Background

Details

For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. ADK5a specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins. The antibody cannot be used for immunoblotting.

Reference

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