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Dot blot with different AAV serotypes and mouse monoclonal anti-AAV5 antibody, clone ADK5a (Courtesy of Regina Heilbronn, Charité Universitätsmedizin Berlin, Mietzsch et al. Hum Gene Ther. 2014 Mar 1; 25(3):212-222) Zoom

Dot blot with different AAV serotypes and mouse monoclonal anti-AAV5 antibody, clone ADK5a (Courtesy of Regina Heilbronn, Charité Universitätsmedizin Berlin, Mietzsch et al. Hum Gene Ther. 2014 Mar 1; 25(3):212-222)

anti-AAV5 (intact particle) mouse monoclonal, ADK5a, lyophilized, purified

Specifically detects intact AAV5 particles, both empty and full capsids. ADK5a recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins. No cross-reaction with other AAV serotypes. The antibody is not suitable for Immunoblotting.


The ADK5a antibody is used as capture and detection antibody in the AAV5 Titration ELISA (PRAAV5).

Cat. No.: 610148
Quantity:  50 µg

Delivery Time: usually 1-7 working days

$427.00
Excl. VAT, excl. Shipping Cost

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Specifications

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anti-AAV5 (intact particle) mouse monoclonal, ADK5a

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anti-AAV5 (intact particle) mouse monoclonal, ADK5a

lyophilized, purified, sample 10 µg
$128.00
Excl. VAT, excl. Shipping Cost

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a kappa
Clone ADK5a
Immunogen AAV5 capsids
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity AAV5

Applications

Tested applications Tested dilutions
Affinity Chromatography assay dependent
Dot Blot assay dependent (non-denaturing conditions)
ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:20
Immunoprecipitation (IP) 1:5
Neutralization Assay assay dependent

Background

Details

For characterization of different stages of infection and very useful for the analysis of the AAV5 assembly process. ADK5a specifically reacts with intact adeno-associated virus 5 particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins. The antibody cannot be used for immunoblotting.
The antibody is usefull for neutralizing experiments.

Limited Use Label License: Research Use Only
Product is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.

Reference

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