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Dot blot with different AAV serotypes and mouse monoclonal anti-AAV5 antibody, clone ADK5a (Courtesy of Regina Heilbronn, Charité Universitätsmedizin Berlin, Mietzsch et al. Hum Gene Ther. 2014 Mar 1; 25(3):212-222) Zoom

Dot blot with different AAV serotypes and mouse monoclonal anti-AAV5 antibody, clone ADK5a (Courtesy of Regina Heilbronn, Charité Universitätsmedizin Berlin, Mietzsch et al. Hum Gene Ther. 2014 Mar 1; 25(3):212-222)

anti-AAV5 (intact particle) mouse monoclonal, ADK5a, supernatant concentrate

Specifically detects intact virus particles, both empty and full capsids. ADK5a recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins. No cross-reaction with other AAV serotypes. The antibody is not suitable for Immunoblotting.
Cat. No.: 652148
Quantity:  0.5 mL

Delivery Time: usually 1-7 working days

€510.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a kappa
Clone ADK5a
Immunogen assembled adeno-associated virus type 5 (AAV5) capsid proteins and virus particles
Purification hybridoma cell culture supernatant concentrate
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity AAV5 (found in human and monkey)
Intended use Research use only

Applications

Tested applications Tested dilutions
Affinity Chromatography assay dependent
ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:100
Immunohistochemistry (IHC) - paraffin 1:100 (microwave treatment recommended)
Immunoprecipitation (IP) assay dependent

Background

Details

For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. ADK5a specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins.

The antibody cannot be used for immunoblotting.

Reference

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